{"id":"W3198539112","doi":"10.1016/j.celrep.2021.109635","title":"Targeted genetic screening in bacteria with a Cas12k-guided transposase","year":2021,"lang":"en","type":"article","venue":"Cell Reports","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":35,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Key Research and Development Program of China; Natural Science Foundation of Guangdong Province; National Natural Science Foundation of China; Simon Fraser University; Harvard Medical School","keywords":"Biology; Transposable element; Genome; Transposon mutagenesis; Computational biology; Genome engineering; Transposase; Mutagenesis; Genetics; Pseudomonas aeruginosa; Gene; Mutant; Genome editing; Bacteria","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009004145,0.0001389507,0.0001350519,0.00003210188,0.00002976386,0.00001773286,0.00004132088,0.00008913434,0.0000482515],"category_scores_gemma":[0.00002561105,0.0001378355,0.00005165275,0.000121491,0.00001878469,0.000001801663,0.00002718772,0.00008486149,0.000001418917],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000005599916,"about_ca_system_score_gemma":0.00009799849,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000313159,"about_ca_topic_score_gemma":0.0001751831,"domain_scores_codex":[0.9990099,0.00002750197,0.0002581116,0.0003792524,0.0000931304,0.0002320836],"domain_scores_gemma":[0.9994311,0.000004421471,0.00005074791,0.0003726397,0.00005466122,0.00008643505],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001757256,0.00003633274,0.01476542,0.00003110387,0.00001607362,0.002986327,0.000068362,0.004779092,0.976486,3.27066e-7,0.0002435358,0.0005699052],"study_design_scores_gemma":[0.0004104871,0.00006305618,0.04949914,0.00002353229,0.00001832401,0.001184377,0.00005147933,0.0001278677,0.9318948,0.000003819868,0.01650015,0.0002229798],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9786129,0.001976776,0.01850091,0.00002780389,0.00009956007,0.0001041456,0.000003127964,0.00001301623,0.0006617643],"genre_scores_gemma":[0.9850554,0.0001060384,0.01377195,0.00009101316,0.00007050859,0.0000148757,0.0001680565,0.00002906995,0.0006931053],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04459115,"threshold_uncertainty_score":0.5620769,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006738469439765006,"score_gpt":0.2465143462882679,"score_spread":0.2397758768485029,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}