{"id":"W3198539112","doi":"10.1016/j.celrep.2021.109635","title":"Targeted genetic screening in bacteria with a Cas12k-guided transposase","year":2021,"lang":"en","type":"article","venue":"Cell Reports","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":35,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Key Research and Development Program of China; Natural Science Foundation of Guangdong Province; National Natural Science Foundation of China; Simon Fraser University; Harvard Medical School","keywords":"Biology; Transposable element; Genome; Transposon mutagenesis; Computational biology; Genome engineering; Transposase; Mutagenesis; Genetics; Pseudomonas aeruginosa; Gene; Mutant; Genome editing; Bacteria","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004289657,0.0008077256,0.0006620747,0.0004361801,0.0003215733,0.0004394944,0.0006941881,0.0005985678,0.001306337],"category_scores_gemma":[0.0003357263,0.0003657847,0.0005999429,0.000329434,0.0003520139,0.0002828988,0.0006756699,0.00068285,0.0008712532],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003618825,"about_ca_system_score_gemma":0.0004948829,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001178575,"about_ca_topic_score_gemma":0.002008351,"domain_scores_codex":[0.9993405,0.0001084945,0.00007647723,0.000157805,0.0002276813,0.00008902933],"domain_scores_gemma":[0.999734,0.0000651504,0.00007481436,0.00005592627,0.00002993592,0.00004020699],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002095048,0.00001344043,0.00006179779,0.00002182571,0.000004349832,0.00003387694,0.000009028048,0.0000318686,0.9988715,0.00005817282,0.00001882161,0.0008542813],"study_design_scores_gemma":[0.000004990549,0.0001151078,0.0005780935,0.000003206825,0.0000106582,0.0001941182,0.0000126071,0.0005889154,0.9975096,0.00002556781,0.0009499344,0.000007197062],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8416931,0.0009812709,0.1493749,0.0002702443,0.00008493119,0.0005338039,0.001566528,0.001933347,0.003561893],"genre_scores_gemma":[0.8441832,0.001490416,0.1430596,0.0001574795,0.00001164253,0.0003679831,0.002188713,0.000390756,0.008150149],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001306337,"threshold_uncertainty_score":0.004370093,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006738469439765006,"score_gpt":0.2465143462882679,"score_spread":0.2397758768485029,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}