{"id":"W3200815404","doi":"10.1002/bip.23475","title":"A simple fluorescent assay for the detection of <scp>p</scp>eptide nucleic acid<scp>‐directed</scp> double strand duplex invasion","year":2021,"lang":"en","type":"article","venue":"Biopolymers","topic":"DNA and Nucleic Acid Chemistry","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Nucleic acid; Peptide nucleic acid; Duplex (building); DNA; Chemistry; Oligonucleotide; Fluorescence; Biochemistry; Nucleic acid thermodynamics; Double stranded; Molecular beacon; A-DNA; Combinatorial chemistry; Molecular biology; Biophysics; Base sequence; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002271986,0.0002706663,0.0002500951,0.00003672339,0.0002209716,0.00004794959,0.0003019611,0.0003265805,0.00002077109],"category_scores_gemma":[0.0004131828,0.0002277837,0.0002952542,0.000215359,0.000184325,0.000009537209,0.000148417,0.0001416008,0.0000070577],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003083437,"about_ca_system_score_gemma":0.0001493144,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006099614,"about_ca_topic_score_gemma":0.0000801713,"domain_scores_codex":[0.9983987,0.0000507637,0.0003425404,0.0005180985,0.0002387458,0.0004512189],"domain_scores_gemma":[0.9987603,0.0001471243,0.000195261,0.0005793722,0.00017041,0.0001474788],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000471501,0.00009804517,0.0003800891,0.0000862453,0.0001670226,0.000003607806,0.0001009203,0.00001148095,0.9899907,0.00001338143,0.005322193,0.003779191],"study_design_scores_gemma":[0.001383081,0.000136385,0.0006211167,0.00002075322,0.00009964214,0.000020364,0.001314082,0.0001993551,0.9269611,0.0000181612,0.06915639,0.00006954526],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9918902,0.004728284,0.001138006,0.00005910732,0.0003350719,0.0003065803,0.0001054603,0.00004779917,0.001389513],"genre_scores_gemma":[0.9964184,0.0005871843,0.0002198348,0.0001485952,0.0003150211,0.00005073224,0.0002913995,0.00005376569,0.001915067],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06383419,"threshold_uncertainty_score":0.928875,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01218155428497956,"score_gpt":0.2357541558853229,"score_spread":0.2235726016003434,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}