{"id":"W3201084854","doi":"10.1186/s12870-021-03138-8","title":"Development of a Csy4-processed guide RNA delivery system with soybean-infecting virus ALSV for genome editing","year":2021,"lang":"en","type":"article","venue":"BMC Plant Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada","funders":"Natural Science Foundation of Hebei Province; Agriculture and Agri-Food Canada; Natural Sciences and Engineering Research Council of Canada; National Natural Science Foundation of China; Iowa State University","keywords":"Biology; Genome editing; Guide RNA; Cas9; CRISPR; Genome; Nicotiana benthamiana; Computational biology; RNA editing; Genetics; Plant virus; Genome engineering; Gene; Virus; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004502446,0.0007365232,0.0004014271,0.0003868557,0.000250687,0.0004037872,0.0005523933,0.0006585462,0.001049678],"category_scores_gemma":[0.000203323,0.0003733186,0.0006525702,0.000195623,0.0002817554,0.0003527285,0.0004682428,0.0009117728,0.0008814936],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006654644,"about_ca_system_score_gemma":0.0005212267,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009291446,"about_ca_topic_score_gemma":0.0009862083,"domain_scores_codex":[0.9995695,0.00003600245,0.00005039349,0.0001207614,0.0001673106,0.00005603515],"domain_scores_gemma":[0.9998411,0.00002046814,0.00005907613,0.00002120509,0.00002429493,0.00003380204],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001701365,0.000008957557,0.00007463388,0.00003749247,0.000004318495,0.00003012435,0.00001152755,0.00008643509,0.9980131,0.000120149,0.00004468339,0.001551531],"study_design_scores_gemma":[0.00001563569,0.0001368444,0.000611073,0.000006904032,0.00001797732,0.0002459756,0.000009656369,0.002075926,0.9898918,0.0000368678,0.006939358,0.00001205675],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6915365,0.002232267,0.2939928,0.0004464258,0.0003282601,0.001153773,0.001912421,0.003051919,0.005345696],"genre_scores_gemma":[0.7671219,0.001387782,0.2118743,0.0001572197,0.00003304756,0.0006335315,0.00566987,0.0004105202,0.01271179],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001049678,"threshold_uncertainty_score":0.004828334,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01443626447027653,"score_gpt":0.2790246146358949,"score_spread":0.2645883501656184,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}