{"id":"W3202548457","doi":"10.3390/v13102002","title":"Macrophage Depletion via Clodronate Pretreatment Reduces Transgene Expression from AAV Vectors In Vivo","year":2021,"lang":"en","type":"article","venue":"Viruses","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Transgene; White pulp; Genetic enhancement; In vivo; Gene delivery; Spleen; Transduction (biophysics); Viral vector; Biology; Immune system; Transfection; Adeno-associated virus; Molecular biology; Cancer research; Cell biology; Immunology; Vector (molecular biology); Gene; Biochemistry; Biotechnology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001118406,0.0001822984,0.0001616807,0.00005360731,0.00006699617,0.00002999365,0.0001639788,0.0001580252,0.0007356363],"category_scores_gemma":[0.00002833828,0.0001718464,0.00008254704,0.0001353557,0.00005155461,0.000007616611,0.0000695506,0.0001011033,0.00003903488],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004550547,"about_ca_system_score_gemma":0.0001060448,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002209691,"about_ca_topic_score_gemma":0.0009880679,"domain_scores_codex":[0.998562,0.0002275424,0.000208095,0.0005009313,0.0002126132,0.0002888648],"domain_scores_gemma":[0.9994078,0.00002124555,0.00004371635,0.0003887748,0.00004760878,0.00009084901],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001019351,0.0001870015,0.002252403,0.000007192713,0.00004863691,0.00005221659,0.0001003825,0.0001377525,0.9913769,6.631585e-7,0.0003069185,0.004510545],"study_design_scores_gemma":[0.0008711609,0.0005671108,0.001467477,0.0000304923,0.000009759014,0.000003338869,0.00004891094,0.00002532632,0.9913649,0.0000483818,0.005384659,0.0001784855],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9922536,0.006940036,0.0001409865,0.00007117695,0.000131467,0.0001790283,0.0001355295,0.00001918201,0.0001289846],"genre_scores_gemma":[0.996981,0.001682624,0.000405504,0.0002445693,0.0001660877,0.00005921365,0.0001725012,0.00003807448,0.0002503809],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005257412,"threshold_uncertainty_score":0.8054701,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02118776969689968,"score_gpt":0.2981721221687924,"score_spread":0.2769843524718927,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}