{"id":"W3202952473","doi":"10.3390/v13101925","title":"Comparison of CRISPR–Cas9 Tools for Transcriptional Repression and Gene Disruption in the BEVS","year":2021,"lang":"en","type":"article","venue":"Viruses","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"CRISPR; Recombineering; Cas9; Biology; Genome editing; Gene; Psychological repression; CRISPR interference; Regulation of gene expression; Genetics; Reporter gene; RNA interference; Computational biology; Genome; Gene expression; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000760978,0.0005307498,0.0006272789,0.001119638,0.0003771218,0.001269024,0.0005886905,0.0006207766,0.0008319542],"category_scores_gemma":[0.0007507899,0.0002768523,0.0005432203,0.0004123421,0.0002831109,0.0006151848,0.0004984029,0.000961834,0.0004694392],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005953753,"about_ca_system_score_gemma":0.0003692403,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008116293,"about_ca_topic_score_gemma":0.001286683,"domain_scores_codex":[0.9988843,0.0001569737,0.0002042917,0.0001529743,0.0004828924,0.0001186394],"domain_scores_gemma":[0.9996165,0.0001386126,0.00007120903,0.00004641028,0.00007316251,0.00005397449],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001894936,0.0000707119,0.0003139209,0.0002348649,0.00003272868,0.0001449636,0.00004580785,0.0006641264,0.9890791,0.0008651537,0.0001690064,0.008190123],"study_design_scores_gemma":[0.00003211376,0.0006718743,0.003299478,0.00004441286,0.00006624404,0.0008572956,0.00008886694,0.003468284,0.9738147,0.0002534398,0.01736367,0.00003967634],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8852605,0.01492261,0.07857802,0.000372909,0.0003723301,0.000627793,0.001946468,0.001090957,0.01682844],"genre_scores_gemma":[0.9103859,0.01032312,0.06716567,0.0001625965,0.00002915098,0.0004336817,0.004790246,0.000346161,0.00636348],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001269024,"threshold_uncertainty_score":0.004319787,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07482983922483222,"score_gpt":0.390342750784341,"score_spread":0.3155129115595088,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}