{"id":"W3202952473","doi":"10.3390/v13101925","title":"Comparison of CRISPR–Cas9 Tools for Transcriptional Repression and Gene Disruption in the BEVS","year":2021,"lang":"en","type":"article","venue":"Viruses","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"CRISPR; Recombineering; Cas9; Biology; Genome editing; Gene; Psychological repression; CRISPR interference; Regulation of gene expression; Genetics; Reporter gene; RNA interference; Computational biology; Genome; Gene expression; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008835264,0.00006731056,0.00009628428,0.00001659839,0.00006464862,0.00002841196,0.00005793196,0.00006560353,0.00001589503],"category_scores_gemma":[0.0001126832,0.00004982572,0.00005942599,0.00004592715,0.00003755475,0.000008657414,0.00002665712,0.00003592864,4.191214e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000002809406,"about_ca_system_score_gemma":0.00002671707,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001590054,"about_ca_topic_score_gemma":0.00003778114,"domain_scores_codex":[0.9994266,0.00006252607,0.0001681092,0.0001793721,0.00007800907,0.00008538344],"domain_scores_gemma":[0.9996739,0.00004044244,0.00005071845,0.0001619903,0.00005065569,0.00002226893],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001205041,0.0001509451,0.012028,0.00002946262,0.00001132565,6.650124e-7,0.000119606,0.0001243221,0.9817677,0.0002518437,0.001951526,0.003444144],"study_design_scores_gemma":[0.0004813403,0.0001928122,0.0289653,0.00002623933,0.00001710545,0.000007093614,0.0004117448,0.00007379873,0.9496947,0.0002918066,0.01975692,0.00008117083],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.992557,0.004192108,0.002714729,0.00009089379,0.0001045109,0.0001373122,0.00008449356,0.000003369131,0.0001155696],"genre_scores_gemma":[0.9987188,0.0002992766,0.0002863889,0.0003878665,0.0001054219,0.00005020357,0.0001174852,0.000006267034,0.00002827765],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03207299,"threshold_uncertainty_score":0.2031834,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07482983922483222,"score_gpt":0.390342750784341,"score_spread":0.3155129115595088,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}