{"id":"W3203868028","doi":"10.1038/s41434-021-00297-z","title":"CRISPR-SCReT (CRISPR-Stop Codon Read Through) method to control Cas9 expression for gene editing","year":2021,"lang":"en","type":"article","venue":"Gene Therapy","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université Laval","funders":"Canadian Institutes of Health Research; Ataxia UK; Government of Canada; Ataxia Canada; National Ataxia Foundation","keywords":"CRISPR; Cas9; Biology; Gene; Exon; Genome editing; Stop codon; Plasmid; Molecular biology; Genetics; Nonsense mutation; Guide RNA; HEK 293 cells; Mutation; Missense mutation","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008006659,0.0008025714,0.001076766,0.001054164,0.0008086578,0.000750423,0.0008889227,0.001090309,0.006388251],"category_scores_gemma":[0.0004707829,0.000510533,0.0009256727,0.0007826635,0.0005386386,0.0004704875,0.0007324905,0.002468595,0.004383362],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006305015,"about_ca_system_score_gemma":0.0007477411,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009519841,"about_ca_topic_score_gemma":0.0024161,"domain_scores_codex":[0.9989533,0.0001192599,0.0001191227,0.000307593,0.0003862358,0.0001145031],"domain_scores_gemma":[0.9997174,0.0000501245,0.00007350637,0.00006697518,0.00004641019,0.00004557504],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007120979,0.00005330998,0.0001105756,0.0001660326,0.00002153569,0.0001531148,0.00003845029,0.0001644741,0.9867458,0.001315395,0.001528922,0.009631186],"study_design_scores_gemma":[0.00002330271,0.00007582246,0.0004259786,0.0000107354,0.00003485135,0.0006275639,0.00001179949,0.001573487,0.972983,0.0001913066,0.02401283,0.00002930205],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1679651,0.0033428,0.7750859,0.00100888,0.001150062,0.002086213,0.006481831,0.01392553,0.02895366],"genre_scores_gemma":[0.4514569,0.002700978,0.4267675,0.0009150576,0.00008544877,0.001554554,0.01139423,0.002150581,0.1029746],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.006388251,"threshold_uncertainty_score":0.02137083,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01898478030847983,"score_gpt":0.36636590565149,"score_spread":0.3473811253430101,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}