{"id":"W3203868028","doi":"10.1038/s41434-021-00297-z","title":"CRISPR-SCReT (CRISPR-Stop Codon Read Through) method to control Cas9 expression for gene editing","year":2021,"lang":"en","type":"article","venue":"Gene Therapy","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université Laval","funders":"Canadian Institutes of Health Research; Ataxia UK; Government of Canada; Ataxia Canada; National Ataxia Foundation","keywords":"CRISPR; Cas9; Biology; Gene; Exon; Genome editing; Stop codon; Plasmid; Molecular biology; Genetics; Nonsense mutation; Guide RNA; HEK 293 cells; Mutation; Missense mutation","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0003448564,0.0002770642,0.000309237,0.00003449312,0.000146292,0.00004292035,0.0002136678,0.0002043495,0.00007068556],"category_scores_gemma":[0.00009017633,0.0002711735,0.0001938044,0.0001168461,0.00001823198,0.000005605824,0.00007884,0.00009766829,0.000007375211],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001981729,"about_ca_system_score_gemma":0.00006905128,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002442827,"about_ca_topic_score_gemma":0.00001281434,"domain_scores_codex":[0.9983242,0.0001121538,0.0003070052,0.0006210693,0.0001666968,0.0004688975],"domain_scores_gemma":[0.9989418,0.00004999111,0.00007259472,0.0005794507,0.0002162028,0.0001399461],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002248974,0.00004065822,0.00004571387,0.00001725886,0.00008128762,0.000008252675,0.0002611875,0.002283129,0.9765162,0.00002035109,0.006000418,0.01450058],"study_design_scores_gemma":[0.001551027,0.0002824741,0.0001054189,0.00001699338,0.00001943724,0.00003568906,0.0001527077,0.0002330029,0.8104405,0.00009254515,0.1868025,0.00026764],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1240656,0.00735471,0.8665589,0.0006451468,0.0005330886,0.0004672524,0.0001317102,0.00003773806,0.0002058047],"genre_scores_gemma":[0.5237672,0.002116158,0.462302,0.004700902,0.004192407,0.000409454,0.0006291256,0.0001898186,0.001692919],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.4042569,"threshold_uncertainty_score":0.9999741,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01898478030847983,"score_gpt":0.36636590565149,"score_spread":0.3473811253430101,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}