{"id":"W3204025012","doi":"10.1016/j.xpro.2021.100794","title":"CloneSeq - Single-cell clonal 3D culture and analysis protocol","year":2021,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"H2020 European Research Council; Israeli Centers for Research Excellence; Azrieli Foundation; Israel Science Foundation; European Research Council; Horizon 2020 Framework Programme; FP7 Coherent Development of Research Policies","keywords":"Protocol (science); Computational biology; Adaptation (eye); Sensitivity (control systems); Clonal selection; Biology; Cell; Computer science; Cell biology; Genetics; Immunology; Medicine; Neuroscience; Engineering","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003968323,0.001857152,0.003084283,0.002449542,0.002754685,0.002849297,0.004058906,0.001696019,0.04492264],"category_scores_gemma":[0.004322204,0.002644138,0.002020053,0.001621423,0.001182195,0.001102604,0.002569685,0.00552368,0.05108792],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001321891,"about_ca_system_score_gemma":0.003242098,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003872254,"about_ca_topic_score_gemma":0.009185519,"domain_scores_codex":[0.996299,0.0005645391,0.0003343165,0.0009551269,0.00148887,0.000358156],"domain_scores_gemma":[0.9976757,0.0006437065,0.00009987665,0.000636248,0.0007464185,0.0001980277],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000818204,0.0002490202,0.001684073,0.002487968,0.0002887025,0.0006120091,0.0007441119,0.00237766,0.7407662,0.01030888,0.190986,0.04867722],"study_design_scores_gemma":[0.0002144941,0.0002930636,0.004195319,0.0002334307,0.0001700932,0.001037134,0.0001251495,0.01051715,0.3635426,0.004147477,0.6151364,0.0003876142],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.01613372,0.002125771,0.7261527,0.000751393,0.001344885,0.007803396,0.1754259,0.04363571,0.02662653],"genre_scores_gemma":[0.02712707,0.00184428,0.6262734,0.001727748,0.0002289942,0.02482116,0.2550802,0.01992108,0.04297604],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.04492264,"threshold_uncertainty_score":0.1502813,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02078226811624943,"score_gpt":0.2850245002695352,"score_spread":0.2642422321532858,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}