{"id":"W3204050772","doi":"10.1016/j.xpro.2021.100863","title":"Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy","year":2021,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Polysaccharides and Plant Cell Walls","field":"Agricultural and Biological Sciences","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; University of Melbourne; International Max Planck Research School for Environmental, Cellular and Molecular Microbiology; Canada Foundation for Innovation; Ontario Research Foundation","keywords":"Cellulose; Arabidopsis; Spinning; Troubleshooting; Microscopy; Membrane; Visualization; Chemistry; Hypocotyl; Sample preparation; Biochemistry; Biophysics; Nanotechnology; Materials science; Biology; Computer science; Botany; Chromatography; Physics; Gene; Data mining; Optics; Polymer chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002627905,0.0001157448,0.0003383827,0.00003695852,0.00005601117,0.00004474124,0.000142228,0.00007514287,0.0007499999],"category_scores_gemma":[0.00004277011,0.00005260455,0.0001642508,0.001239285,0.00003091006,0.0000980431,0.0000695605,0.0001058911,0.000003670413],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003137776,"about_ca_system_score_gemma":0.00002487303,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001947665,"about_ca_topic_score_gemma":0.001577884,"domain_scores_codex":[0.9989597,0.0001185259,0.0002367641,0.0002842509,0.0001541769,0.0002465518],"domain_scores_gemma":[0.9995347,0.0001333138,0.0001391703,0.00008464687,0.00004474151,0.00006341225],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004820014,0.0001484372,0.05209829,0.00002092298,0.0000593801,0.00003122126,0.00004909483,0.0000627035,0.9411011,0.00001145356,0.000007057539,0.006362184],"study_design_scores_gemma":[0.0001502219,0.00007918768,0.1387388,0.0001259306,0.00009516194,0.000001862463,0.00008499793,0.003310306,0.8560566,0.00004886277,0.001117516,0.0001905411],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9939902,0.00002561725,0.00001882975,0.00005327382,0.00001484511,0.005572512,0.0001883941,0.00001178016,0.0001245221],"genre_scores_gemma":[0.9979838,0.000004696074,0.000438385,0.00003308111,0.00002959119,0.001387565,0.00003443472,8.137167e-7,0.00008760164],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.08664055,"threshold_uncertainty_score":0.8211973,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04386548209916431,"score_gpt":0.307015180307503,"score_spread":0.2631496982083387,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}