{"id":"W3205944505","doi":"10.1016/j.talanta.2021.122981","title":"Fluorescein isothiocyanate, a platform for the selective and sensitive detection of S-Nitrosothiols and hydrogen sulfide","year":2021,"lang":"en","type":"article","venue":"Talanta","topic":"Sulfur Compounds in Biology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Windsor","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Fluorescein; Hydrogen sulfide; Dithiothreitol; Glutathione; Fluorescein isothiocyanate; Cysteine; Isothiocyanate; Quenching (fluorescence); Intracellular; Biochemistry; Fluorescence; Organic chemistry; Enzyme; Sulfur","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003387662,0.0006933484,0.0002855291,0.0004829604,0.0003861148,0.0003302695,0.0008551751,0.0008504365,0.001565104],"category_scores_gemma":[0.0002345883,0.000353073,0.0002966224,0.0002703129,0.000355879,0.000408435,0.0003250265,0.0008551812,0.0005888265],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006878531,"about_ca_system_score_gemma":0.0003395411,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001118759,"about_ca_topic_score_gemma":0.002079412,"domain_scores_codex":[0.9997639,0.00003240252,0.000008804765,0.00006367381,0.00007333342,0.00005790279],"domain_scores_gemma":[0.9998393,0.00004270922,0.00003452064,0.00001847903,0.00003003911,0.00003493629],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003958233,0.000007564629,0.00002661183,0.00001687469,0.000002789812,0.00004079685,0.000007499921,0.0000353799,0.9983279,0.000185897,0.0001234467,0.001185649],"study_design_scores_gemma":[0.00000762914,0.0001414669,0.000304984,0.000002409122,0.000008299095,0.0002000624,0.000009674768,0.001090835,0.996601,0.00004583867,0.001582659,0.000005096353],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8029804,0.00245754,0.1800546,0.001108002,0.0002516413,0.0001048737,0.0004936837,0.001778543,0.0107707],"genre_scores_gemma":[0.9271076,0.001580447,0.05854944,0.0004051722,0.00006823712,0.0001022904,0.0006916139,0.00008140227,0.0114138],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001565104,"threshold_uncertainty_score":0.005235791,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01040730083091458,"score_gpt":0.2393892549066648,"score_spread":0.2289819540757502,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}