{"id":"W3206545566","doi":"10.1002/cbic.202100476","title":"Target‐Mediated 5’‐Exonuclease Digestion of DNA Aptamers with RecJ to Modulate Rolling Circle Amplification for Biosensing","year":2021,"lang":"en","type":"article","venue":"ChemBioChem","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Aptamer; Exonuclease; Rolling circle replication; Primer (cosmetics); DNA; Exonuclease III; Biosensor; Digestion (alchemy); Biology; Chemistry; Molecular biology; Biochemistry; Biophysics; DNA polymerase; Chromatography; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003325897,0.0005031487,0.0003661388,0.0002019836,0.0001258463,0.0004226709,0.0003532585,0.0005200348,0.0004048368],"category_scores_gemma":[0.0002514372,0.0002845277,0.0002926072,0.0001616911,0.0002557988,0.0003371313,0.0002298428,0.0006639896,0.0003837301],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002257646,"about_ca_system_score_gemma":0.0001319649,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001476049,"about_ca_topic_score_gemma":0.0002520252,"domain_scores_codex":[0.9995081,0.00005971785,0.00003352818,0.0001361943,0.0002016064,0.00006087268],"domain_scores_gemma":[0.9997898,0.00006090743,0.00006811519,0.00002616116,0.00003098911,0.00002399868],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001296933,0.000009488772,0.00002851776,0.00002018134,0.000002040162,0.00001933534,0.00001269209,0.00005228863,0.9983675,0.00005970672,0.00001841437,0.001396888],"study_design_scores_gemma":[0.000001544713,0.00002913009,0.00008533791,7.703082e-7,0.000001569726,0.00005366127,0.000001751822,0.0005001048,0.9987915,0.000009228164,0.0005229912,0.000002482491],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7981958,0.005143669,0.1921152,0.0003139784,0.0001921276,0.0001770152,0.0002101488,0.0009842654,0.002667767],"genre_scores_gemma":[0.9146726,0.00142402,0.08026859,0.0001504542,0.00003734661,0.0001039862,0.0001973854,0.0001104909,0.003035066],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005200348,"threshold_uncertainty_score":0.001758933,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01078818083039094,"score_gpt":0.2511343622385084,"score_spread":0.2403461814081174,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}