{"id":"W3206545566","doi":"10.1002/cbic.202100476","title":"Target‐Mediated 5’‐Exonuclease Digestion of DNA Aptamers with RecJ to Modulate Rolling Circle Amplification for Biosensing","year":2021,"lang":"en","type":"article","venue":"ChemBioChem","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Aptamer; Exonuclease; Rolling circle replication; Primer (cosmetics); DNA; Exonuclease III; Biosensor; Digestion (alchemy); Biology; Chemistry; Molecular biology; Biochemistry; Biophysics; DNA polymerase; Chromatography; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00014045,0.0001601223,0.0002164857,0.00005698053,0.00007794738,0.00001960763,0.00008389947,0.0001495396,9.791447e-7],"category_scores_gemma":[0.0002627822,0.0001609902,0.0001086117,0.0002963492,0.000070099,0.00000532026,0.00004476381,0.0000541704,0.000001372042],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000258129,"about_ca_system_score_gemma":0.00006785729,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000004703075,"about_ca_topic_score_gemma":0.000006180144,"domain_scores_codex":[0.9988897,0.0000216084,0.0002550822,0.0004877769,0.0001211579,0.0002246714],"domain_scores_gemma":[0.9988695,0.00001862708,0.0001545427,0.0004106213,0.0004455746,0.0001011264],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000161528,0.00006566317,0.000104474,0.00003306207,0.00006218272,0.0000017197,0.00002716016,0.0000566881,0.9953136,0.000009209395,0.0001305981,0.004034085],"study_design_scores_gemma":[0.0003467216,0.0001539858,0.0003127525,0.00006105931,0.00004928206,0.00000890563,0.0001228176,0.0003519091,0.9965311,0.00005438495,0.001779415,0.0002276334],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9683679,0.0001058614,0.03070595,0.0003678503,0.00003372643,0.0002169837,0.00003991799,0.00004742572,0.0001143957],"genre_scores_gemma":[0.9480613,0.00004603061,0.0506865,0.0002008507,0.0000895819,0.00001130634,0.0007294499,0.00003256302,0.0001424472],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02030662,"threshold_uncertainty_score":0.6564988,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01078818083039094,"score_gpt":0.2511343622385084,"score_spread":0.2403461814081174,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}