{"id":"W3207470655","doi":"10.1016/j.ab.2021.114429","title":"Identification of in vitro JMJD lysine demethylase candidate substrates via systematic determination of substrate preference","year":2021,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Epigenetics and DNA Methylation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":false,"ca_institutions":"Carleton University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"H3K4me3; Demethylase; Histone; Histone methylation; Histone H3; Histone methyltransferase; Biology; Biochemistry; Lysine; Proteome; Histone H2A; Methylation; Gene expression; Gene; DNA methylation; Amino acid; Promoter","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008831666,0.0007734992,0.0007193006,0.0003040858,0.0003789365,0.0005933286,0.0004743998,0.0003653372,0.001036222],"category_scores_gemma":[0.001067661,0.0004002689,0.0003695798,0.0005333473,0.0002746457,0.0003132576,0.0003954351,0.0009026311,0.0007723847],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005227487,"about_ca_system_score_gemma":0.0006242483,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001017951,"about_ca_topic_score_gemma":0.002924876,"domain_scores_codex":[0.9994144,0.0001747132,0.0000837269,0.0001341029,0.00009988787,0.0000931999],"domain_scores_gemma":[0.999294,0.0002280045,0.00008854485,0.0001236683,0.0001806284,0.00008517411],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000114058,0.00004225269,0.0004686848,0.00005447361,0.00001498666,0.00006380306,0.00002432169,0.000143282,0.9963576,0.0001150478,0.00005345705,0.002548054],"study_design_scores_gemma":[0.000003349175,0.00004736471,0.0004042328,0.00000211168,0.0000154847,0.00007023628,0.00001114997,0.0003558684,0.9982029,0.00002190158,0.0008604956,0.000004922326],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9313977,0.002409243,0.06087198,0.0002488492,0.00009448981,0.0002066794,0.001452391,0.0003300337,0.002988579],"genre_scores_gemma":[0.9238832,0.002208121,0.0649523,0.0001213876,0.00002245451,0.0001604517,0.004148758,0.0001918781,0.004311488],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001036222,"threshold_uncertainty_score":0.00467068,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01412097400049366,"score_gpt":0.2678053191841553,"score_spread":0.2536843451836617,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}