{"id":"W3207594484","doi":"10.3390/fermentation7040227","title":"Bacillus velezensis Identification and Recombinant Expression, Purification, and Characterization of Its Alpha-Amylase","year":2021,"lang":"en","type":"article","venue":"Fermentation","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lakehead University","funders":"Natural Sciences and Engineering Research Council of Canada; Xuchang University; China Scholarship Council","keywords":"Amylase; Enzyme; Alpha-amylase; Recombinant DNA; Chemistry; Gene; Biochemistry; Signal peptide; Coding region; Bacteria; Biology; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001051927,0.00007639782,0.00007600344,0.00004001646,0.00007538462,0.00003091124,0.00002735566,0.00006519433,0.00002797915],"category_scores_gemma":[0.00006593692,0.00008358299,0.00001512265,0.00009519999,0.00002753684,0.00002707555,0.00002869384,0.00002529683,0.00000304961],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007701708,"about_ca_system_score_gemma":0.00002649235,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":7.442522e-7,"about_ca_topic_score_gemma":9.829797e-7,"domain_scores_codex":[0.9992638,0.00006687629,0.0002420771,0.0002760444,0.00008515957,0.00006610322],"domain_scores_gemma":[0.9993723,0.000002762234,0.0001759393,0.0001522207,0.0002605263,0.00003624087],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002584276,0.00004650015,0.001473736,0.00005109494,0.000009088598,2.275309e-7,0.0001767746,8.163309e-7,0.9921377,0.00009363714,0.00008584165,0.005898799],"study_design_scores_gemma":[0.0002606919,0.00002976576,0.1960988,0.00001475151,0.00001267219,0.00001123913,0.0000979376,0.00004633056,0.8014522,0.00002578954,0.001880467,0.00006936032],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965087,0.0002262244,0.002340888,0.0005203084,0.0001963788,0.0001528573,0.00002176567,0.000008896463,0.00002405185],"genre_scores_gemma":[0.9936934,0.002107421,0.0002285668,0.00008805176,0.00006333966,0.00002649999,0.00247198,0.00001069437,0.001310081],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.194625,"threshold_uncertainty_score":0.3408416,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.010099893372718,"score_gpt":0.2404146325956893,"score_spread":0.2303147392229714,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}