{"id":"W3207594484","doi":"10.3390/fermentation7040227","title":"Bacillus velezensis Identification and Recombinant Expression, Purification, and Characterization of Its Alpha-Amylase","year":2021,"lang":"en","type":"article","venue":"Fermentation","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lakehead University","funders":"Natural Sciences and Engineering Research Council of Canada; Xuchang University; China Scholarship Council","keywords":"Amylase; Enzyme; Alpha-amylase; Recombinant DNA; Chemistry; Gene; Biochemistry; Signal peptide; Coding region; Bacteria; Biology; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001791637,0.0004785239,0.0003701648,0.0003991239,0.0001958236,0.000542582,0.000221286,0.0002114121,0.0003841921],"category_scores_gemma":[0.0003587079,0.0001308494,0.0002890867,0.0005970291,0.0001519465,0.0003520147,0.000429145,0.0004673205,0.0003600949],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002779498,"about_ca_system_score_gemma":0.0003292371,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002785031,"about_ca_topic_score_gemma":0.001744824,"domain_scores_codex":[0.9997908,0.0000209234,0.00002923951,0.00004654019,0.00007455606,0.00003792039],"domain_scores_gemma":[0.9998982,0.00001098835,0.00002599534,0.00001165521,0.00003089459,0.00002232228],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006135434,0.00003452104,0.0017899,0.00008595142,0.000006377777,0.0001839775,0.00005967636,0.0001275644,0.9929217,0.00009093173,0.00005556696,0.004582671],"study_design_scores_gemma":[0.00003313665,0.000319622,0.04253525,0.00007240594,0.00006454453,0.001737997,0.0006654696,0.003685238,0.9375301,0.000269677,0.01306087,0.00002565177],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9828202,0.002480773,0.01115783,0.0003065818,0.00003306483,0.00004847398,0.0007750457,0.0001298876,0.002248219],"genre_scores_gemma":[0.9761211,0.002398817,0.01071108,0.00007833305,0.00001716738,0.00007094154,0.00488743,0.00005275015,0.005662305],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002785031,"threshold_uncertainty_score":0.005537629,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.010099893372718,"score_gpt":0.2404146325956893,"score_spread":0.2303147392229714,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}