{"id":"W3210241331","doi":"10.1007/978-1-0716-1720-5_4","title":"Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen Candida albicans","year":2021,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Antifungal resistance and susceptibility","field":"Medicine","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Candida albicans; CRISPR; Biology; CRISPR interference; Gene; Cas9; Psychological repression; Computational biology; Genetics; Plasmid; Genome editing; RNA interference; RNA; Gene expression","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005581811,0.000612872,0.000537501,0.0006479624,0.000480943,0.0007959082,0.001104646,0.0006337228,0.001406331],"category_scores_gemma":[0.0003910081,0.0005609847,0.0005754387,0.0003333725,0.0004860008,0.0002399146,0.0004951452,0.001449578,0.0006361315],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006961262,"about_ca_system_score_gemma":0.0007330886,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001125281,"about_ca_topic_score_gemma":0.002031202,"domain_scores_codex":[0.999364,0.00006707862,0.00008637398,0.0001330435,0.000262498,0.00008710854],"domain_scores_gemma":[0.9996935,0.00006507647,0.00008514854,0.00005006718,0.00004172113,0.00006460467],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005556784,0.00003478518,0.0001160082,0.00003632174,0.000007867964,0.00006126598,0.00002301382,0.0003416286,0.9961936,0.0006524681,0.0001108865,0.002366609],"study_design_scores_gemma":[0.00004071262,0.0001425257,0.0008772541,0.000009214737,0.00002896977,0.0001977545,0.0000230996,0.004011258,0.987371,0.0001449905,0.007133284,0.0000200098],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6432077,0.001271858,0.3389781,0.0007940035,0.0003054308,0.001497199,0.004021394,0.002543534,0.007380811],"genre_scores_gemma":[0.838084,0.0008280983,0.1489562,0.0002881117,0.00003001054,0.0005847292,0.003431292,0.0003575089,0.007440024],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001406331,"threshold_uncertainty_score":0.005050719,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0447028395463388,"score_gpt":0.4001730928751208,"score_spread":0.355470253328782,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}