{"id":"W3210749319","doi":"10.1128/aem.01485-21","title":"Golden Gate Assembly of Aerobic and Anaerobic Microbial Bioreporters","year":2021,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada; Government of Canada","keywords":"Bioreporter; Biosensor; Reporter gene; Escherichia coli; Synthetic biology; Biology; Green fluorescent protein; Analyte; Computational biology; Gene; Chemistry; Genetics; Biochemistry; Gene expression","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002521204,0.0004085077,0.0004228216,0.0001875049,0.0002882484,0.0006343691,0.0005510757,0.0005333013,0.0006165701],"category_scores_gemma":[0.00030814,0.0003261359,0.000363524,0.0001813662,0.0003284023,0.0004150789,0.0006533123,0.000628548,0.0004195205],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005269056,"about_ca_system_score_gemma":0.0002312852,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003472505,"about_ca_topic_score_gemma":0.0005637318,"domain_scores_codex":[0.9996735,0.00003993717,0.000026049,0.00008569099,0.00009472076,0.00008007132],"domain_scores_gemma":[0.9998517,0.00002125011,0.00003189196,0.00001914042,0.00002146739,0.00005454403],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008251899,0.00003166631,0.0001783279,0.00003505766,0.000009412587,0.0002286493,0.00005386702,0.002067792,0.990432,0.004038402,0.0001699952,0.002672423],"study_design_scores_gemma":[0.00002536519,0.0001911163,0.0004044705,0.00000737523,0.00001028761,0.0001150435,0.00003824729,0.0198835,0.9720486,0.0006776685,0.006575741,0.00002263651],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9310412,0.0005229216,0.06175674,0.0002920469,0.0002030856,0.00007066444,0.0002261452,0.0006296168,0.005257556],"genre_scores_gemma":[0.9599191,0.0003942797,0.03450488,0.00008843305,0.00001935013,0.00007420671,0.0004519189,0.00009671211,0.004451167],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006343691,"threshold_uncertainty_score":0.003822982,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005265249110798779,"score_gpt":0.2014953811538629,"score_spread":0.1962301320430641,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}