{"id":"W3212233672","doi":"10.1038/s41467-021-26989-z","title":"Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles","year":2021,"lang":"en","type":"article","venue":"Nature Communications","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":35,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"National Institute of General Medical Sciences; National Institutes of Health; Foundation for the National Institutes of Health","keywords":"Pseudoknot; CRISPR; RNA; Computational biology; Genome editing; Trans-activating crRNA; Nucleotide; Ribose; Gene; Cas9; Chemistry; CRISPR interference; Biology; Combinatorial chemistry; Biochemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002766452,0.0005822137,0.0004726785,0.0002699683,0.0002609845,0.0005186719,0.0004671145,0.0005295961,0.001299103],"category_scores_gemma":[0.0003958929,0.0003601144,0.0004593659,0.0002385074,0.0003688044,0.000237822,0.0004049848,0.0009179705,0.001059235],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003654625,"about_ca_system_score_gemma":0.0003289892,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005632723,"about_ca_topic_score_gemma":0.001396716,"domain_scores_codex":[0.9995198,0.00007116509,0.00004984677,0.0001217835,0.000185039,0.00005233411],"domain_scores_gemma":[0.9995885,0.00007366511,0.000151119,0.00008917678,0.00004543096,0.00005215002],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004747009,0.000009498565,0.00007414247,0.00004094036,0.000007683345,0.00006901136,0.00001762039,0.0004607337,0.9964125,0.0003573793,0.00006486499,0.002438058],"study_design_scores_gemma":[0.000007210823,0.0001259851,0.0002768173,0.000003291089,0.00001034196,0.0002279043,0.000005683672,0.001080164,0.9930779,0.0001011886,0.005072242,0.00001134897],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7572338,0.001559648,0.2281183,0.0002129748,0.0002418763,0.0003649055,0.001174033,0.002718639,0.008375888],"genre_scores_gemma":[0.8501821,0.001030406,0.1341788,0.0002065173,0.00003173231,0.0001797165,0.001582156,0.0004674369,0.01214116],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001299103,"threshold_uncertainty_score":0.004345953,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01653850492674452,"score_gpt":0.3439751000443849,"score_spread":0.3274365951176404,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}