{"id":"W3212233672","doi":"10.1038/s41467-021-26989-z","title":"Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles","year":2021,"lang":"en","type":"article","venue":"Nature Communications","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":35,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"National Institute of General Medical Sciences; National Institutes of Health; Foundation for the National Institutes of Health","keywords":"Pseudoknot; CRISPR; RNA; Computational biology; Genome editing; Trans-activating crRNA; Nucleotide; Ribose; Gene; Cas9; Chemistry; CRISPR interference; Biology; Combinatorial chemistry; Biochemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001216598,0.0001492975,0.0001282503,0.00004059376,0.0002947773,0.00007401789,0.0004809333,0.0002405479,0.000007003664],"category_scores_gemma":[0.0002104707,0.000142662,0.00006515898,0.0001958201,0.00008653729,0.000006026932,0.0003356489,0.0003959109,0.000002873852],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001422181,"about_ca_system_score_gemma":0.0001326021,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001244953,"about_ca_topic_score_gemma":0.000437187,"domain_scores_codex":[0.9991642,0.00006242457,0.000184101,0.0002622494,0.0001202153,0.0002068524],"domain_scores_gemma":[0.998064,0.00004306318,0.00006138968,0.001470079,0.0002926376,0.00006877091],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001743215,0.0001184311,0.002392378,0.00002563299,0.0001361623,0.00001002516,0.0001466919,0.001100083,0.9871992,0.001439681,0.00380103,0.003613255],"study_design_scores_gemma":[0.0005508569,0.00005541231,0.004959696,0.00006883338,0.00007006255,0.0001744043,0.0003466491,0.0006563815,0.9037324,0.00006553552,0.08898035,0.000339419],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6974141,0.135014,0.1096668,0.009595383,0.0009545179,0.0005391908,0.0001601696,0.0002336459,0.04642225],"genre_scores_gemma":[0.9473548,0.001152898,0.0501132,0.0003432159,0.000269213,0.0000218655,0.0004775305,0.00002946165,0.0002378346],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2499407,"threshold_uncertainty_score":0.5817586,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01653850492674452,"score_gpt":0.3439751000443849,"score_spread":0.3274365951176404,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}