{"id":"W3213113520","doi":"10.3791/62448","title":"Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells","year":2021,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"","keywords":"Receptor; Peptide; HEK 293 cells; G protein-coupled receptor; Cell biology; Calcium in biology; Cell culture; In vitro; Chemistry; Biology; Molecular biology; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003611674,0.0005927648,0.000652278,0.0003623437,0.0002041074,0.0005856184,0.000437803,0.0005644304,0.001685639],"category_scores_gemma":[0.0003128072,0.0002981594,0.0004043639,0.0005757737,0.0001869688,0.0003207294,0.000393506,0.0006760867,0.00159033],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002339082,"about_ca_system_score_gemma":0.0001578141,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002006101,"about_ca_topic_score_gemma":0.0003632432,"domain_scores_codex":[0.999442,0.00009302647,0.00008403761,0.0000976828,0.0002107626,0.00007244038],"domain_scores_gemma":[0.999869,0.00004187748,0.00002654826,0.00001891945,0.00002932181,0.00001429042],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006273483,0.00003854332,0.0000934632,0.0000542746,0.000005425129,0.00005149586,0.00001249855,0.0002317593,0.9977071,0.00005313172,0.00004746845,0.001642007],"study_design_scores_gemma":[0.00001858033,0.0003158042,0.0004369055,0.000003978802,0.00001782193,0.0001899965,0.00001912112,0.001480306,0.9943537,0.00002700896,0.003129281,0.00000754166],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9066101,0.003284758,0.07684059,0.0003481252,0.0001983136,0.0009183217,0.004260655,0.000578909,0.00696036],"genre_scores_gemma":[0.8464473,0.004449186,0.1195509,0.000388505,0.00005402114,0.001031979,0.01042024,0.0002005376,0.0174574],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001685639,"threshold_uncertainty_score":0.005639076,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04572878765413672,"score_gpt":0.3878261915230718,"score_spread":0.3420974038689351,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}