{"id":"W3213324499","doi":"10.1016/j.xpro.2021.100956","title":"Production of human CAR-NK cells with lentiviral vectors and functional assessment in vitro","year":2021,"lang":"en","type":"article","venue":"STAR Protocols","topic":"CAR-T cell therapy research","field":"Medicine","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Canadian Institutes of Health Research","keywords":"Chimeric antigen receptor; Flow cytometry; Cell biology; Ex vivo; Transgene; Transduction (biophysics); Janus kinase 3; Biology; In vitro; Immune system; Effector; Interleukin 21; T cell; Immunology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00176544,0.001022617,0.0009588671,0.0007047165,0.0004676493,0.001548558,0.0009752946,0.0008044001,0.004947127],"category_scores_gemma":[0.0006554751,0.0006607264,0.0006372639,0.0006760548,0.0004510375,0.0006316506,0.0006378268,0.002627793,0.004607715],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009344197,"about_ca_system_score_gemma":0.0006906445,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001587059,"about_ca_topic_score_gemma":0.001745136,"domain_scores_codex":[0.998457,0.0002932535,0.0002705409,0.000212135,0.0005084785,0.0002585774],"domain_scores_gemma":[0.9995402,0.0001193475,0.00003990657,0.000134324,0.0001132877,0.00005296057],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001716118,0.0002591829,0.000252002,0.0002506875,0.00002154769,0.00009621108,0.0001788762,0.000810991,0.9881557,0.001439472,0.001498451,0.00686531],"study_design_scores_gemma":[0.00007140166,0.0003120005,0.0006803737,0.00003075276,0.00003698141,0.0002296869,0.00003358319,0.003474038,0.9740697,0.0002011541,0.02083802,0.00002243567],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6520012,0.01144437,0.2579207,0.001094508,0.001042993,0.005665286,0.02732311,0.004411231,0.03909657],"genre_scores_gemma":[0.7966559,0.006250367,0.1188029,0.0005557203,0.0001562517,0.005029025,0.0385272,0.0007774216,0.03324529],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.004947127,"threshold_uncertainty_score":0.01654977,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04441661667281394,"score_gpt":0.3650674394608381,"score_spread":0.3206508227880242,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}