{"id":"W3214148889","doi":"10.1042/bst20210700","title":"Directed evolution approaches for optogenetic tool development","year":2021,"lang":"en","type":"article","venue":"Biochemical Society Transactions","topic":"Photochromic and Fluorescence Chemistry","field":"Materials Science","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Optogenetics; Directed evolution; Directed Molecular Evolution; Biological evolution; Synthetic biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000774847,0.0007569646,0.0004766751,0.0007296083,0.0003950711,0.0009294098,0.0009464158,0.0009427759,0.001849838],"category_scores_gemma":[0.0004907426,0.0005674033,0.000492067,0.0006248662,0.0006618788,0.0007628207,0.001006315,0.001903518,0.001216923],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001249888,"about_ca_system_score_gemma":0.0004652627,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000555553,"about_ca_topic_score_gemma":0.001198728,"domain_scores_codex":[0.9994928,0.00009193446,0.00004071486,0.00009301922,0.0002282372,0.00005321575],"domain_scores_gemma":[0.9998524,0.00004819106,0.00003448188,0.00001990457,0.00002881703,0.00001621673],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005559292,0.0001116754,0.000319702,0.00105276,0.00006979974,0.0002805787,0.000170591,0.003298009,0.7900174,0.06267922,0.002331326,0.1396134],"study_design_scores_gemma":[0.00006618502,0.000340549,0.0006039612,0.0002544197,0.00009503351,0.001462036,0.0000778471,0.01029876,0.5971843,0.01506138,0.3744667,0.00008886875],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.04780561,0.09454972,0.8191245,0.002297375,0.0007519163,0.0006625072,0.0004424362,0.0009890792,0.0333768],"genre_scores_gemma":[0.1803334,0.1124747,0.6902224,0.001135255,0.0001460675,0.001104817,0.0006821748,0.0002264158,0.01367479],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001849838,"threshold_uncertainty_score":0.009068668,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02933833070420286,"score_gpt":0.2302668904714716,"score_spread":0.2009285597672687,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}