{"id":"W3214148889","doi":"10.1042/bst20210700","title":"Directed evolution approaches for optogenetic tool development","year":2021,"lang":"en","type":"article","venue":"Biochemical Society Transactions","topic":"Photochromic and Fluorescence Chemistry","field":"Materials Science","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Optogenetics; Directed evolution; Directed Molecular Evolution; Biological evolution; Synthetic biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0000894904,0.0001252587,0.0001332801,0.000004901891,0.000245877,0.00003581333,0.0001191234,0.0001630703,0.0003584905],"category_scores_gemma":[0.00002180778,0.0001276707,0.0001982824,0.0001388279,0.0001082493,0.00005271255,0.00000986151,0.00008663719,0.00002001043],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001994498,"about_ca_system_score_gemma":0.0002783616,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003066172,"about_ca_topic_score_gemma":8.014782e-7,"domain_scores_codex":[0.9990065,0.00001037947,0.0002067391,0.0003573971,0.0001474209,0.0002715862],"domain_scores_gemma":[0.9995899,0.00004469056,0.00003332487,0.0001694315,0.00007917554,0.00008346105],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001065562,0.0001343288,0.00001144344,0.00006554046,0.0000211216,2.139761e-7,0.0002023187,0.0000212683,0.9978962,0.00001713561,0.001142544,0.0004772538],"study_design_scores_gemma":[0.000322185,0.000004728179,0.0002439698,0.00001918482,0.00003117439,0.000007855874,0.0002331577,0.001111455,0.9960769,0.00008609393,0.001682702,0.0001806001],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6226413,0.00030931,0.3763579,0.0001245756,0.0001089759,0.0001707735,0.00005833063,0.0001441736,0.00008461961],"genre_scores_gemma":[0.8924241,0.00001467979,0.1065843,0.00005578183,0.00007848314,0.0002653369,0.00008758555,0.00001376327,0.0004759475],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2697828,"threshold_uncertainty_score":0.5206262,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02933833070420286,"score_gpt":0.2302668904714716,"score_spread":0.2009285597672687,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}