{"id":"W3214527427","doi":"10.1007/978-1-0716-1900-1_5","title":"Two-Dimensional Electrophoresis Coupled with Western Blot as a Method to Detect Potential Neutralizing Antibody Targets from Gram-Negative Intracellular Bacteria","year":2021,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Bacteriophages and microbial interactions","field":"Environmental Science","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan; Université de Montréal","funders":"","keywords":"Western blot; Antibody; Bacteria; Blot; Antigen; Biology; Intracellular; Protein subunit; Gel electrophoresis; Virology; Neutralizing antibody; Microbiology; Molecular biology; Cell biology; Immunology; Gene; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001371687,0.003178372,0.001231478,0.002702632,0.001141646,0.001186519,0.002124615,0.001033373,0.003166633],"category_scores_gemma":[0.0008088782,0.001013588,0.001335602,0.00160317,0.0009859045,0.001285929,0.0008135859,0.003877169,0.002266936],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006317255,"about_ca_system_score_gemma":0.0006432447,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009202799,"about_ca_topic_score_gemma":0.001767599,"domain_scores_codex":[0.9984596,0.0004123383,0.000219402,0.0003494267,0.0003432933,0.0002159759],"domain_scores_gemma":[0.9987943,0.0004984459,0.0001491649,0.0001815928,0.0002637532,0.0001126975],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009800687,0.0000976472,0.00008158325,0.0001874429,0.00002607622,0.00002888486,0.00005025489,0.00002790296,0.9973224,0.0002417096,0.0001712053,0.001667],"study_design_scores_gemma":[0.00005796607,0.0003820974,0.003550108,0.0000456402,0.0001391283,0.0007399176,0.00008649861,0.003848695,0.9837164,0.0006097304,0.006777082,0.00004687588],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2058973,0.01228238,0.7633272,0.001941568,0.001726917,0.0006916879,0.002268627,0.003534754,0.008329527],"genre_scores_gemma":[0.2540744,0.01563886,0.6981738,0.001432277,0.0005635827,0.002463347,0.008575816,0.001102012,0.01797599],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003178372,"threshold_uncertainty_score":0.01059347,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008204540351736811,"score_gpt":0.3429673579371784,"score_spread":0.3347628175854416,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}