{"id":"W379593536","doi":"10.3791/52941","title":"qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Cancer Institute; Natural Sciences and Engineering Research Council of Canada; Advanced Research Projects Agency; National Science Foundation","keywords":"Genotyping; Genome; Amplicon; Biology; Chromosome; Genetics; Molecular biology; Agarose gel electrophoresis; Computational biology; Primer (cosmetics); ORFS; DNA; Polymerase chain reaction; Gene; Open reading frame; Chemistry; Genotype","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.004093021,0.002281093,0.002043252,0.002733313,0.0007740399,0.001614755,0.002089028,0.001439094,0.007849239],"category_scores_gemma":[0.003929649,0.001714864,0.001444721,0.001791074,0.001322063,0.001021248,0.00128561,0.003189656,0.01219508],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007806906,"about_ca_system_score_gemma":0.001303411,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007116473,"about_ca_topic_score_gemma":0.001980718,"domain_scores_codex":[0.9934992,0.001293735,0.0004024715,0.001866866,0.002499139,0.0004387232],"domain_scores_gemma":[0.9967367,0.001203135,0.0004330468,0.0006080584,0.0007847386,0.0002343556],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001097377,0.00006866463,0.0005451748,0.0002537723,0.00002223817,0.00004927997,0.00005759358,0.0003414375,0.9879226,0.000637161,0.00224222,0.007750085],"study_design_scores_gemma":[0.00004542611,0.0002330601,0.002368088,0.00005647271,0.00005959295,0.000236219,0.00003966022,0.006911478,0.9595346,0.000646999,0.02980325,0.00006512341],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.02405437,0.0007247743,0.9448429,0.0002588487,0.0003105735,0.00136015,0.01287015,0.01191455,0.003663724],"genre_scores_gemma":[0.06085423,0.00172475,0.8759117,0.0006638162,0.0001465995,0.005824842,0.03317674,0.004566909,0.01713045],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.007849239,"threshold_uncertainty_score":0.02625835,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04917753157794701,"score_gpt":0.4418327896324559,"score_spread":0.3926552580545089,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}