{"id":"W379593536","doi":"10.3791/52941","title":"qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Cancer Institute; Natural Sciences and Engineering Research Council of Canada; Advanced Research Projects Agency; National Science Foundation","keywords":"Genotyping; Genome; Amplicon; Biology; Chromosome; Genetics; Molecular biology; Agarose gel electrophoresis; Computational biology; Primer (cosmetics); ORFS; DNA; Polymerase chain reaction; Gene; Open reading frame; Chemistry; Genotype","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000885949,0.0001727313,0.0003877754,0.0001837433,0.00007534467,0.00005895847,0.0003239044,0.0001458782,0.0000669775],"category_scores_gemma":[0.000183563,0.0001444591,0.0003431365,0.0002539575,0.0000533543,0.00001046072,0.0001317217,0.00009426747,0.00001878499],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006233384,"about_ca_system_score_gemma":0.0002285139,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004141768,"about_ca_topic_score_gemma":0.0000028547,"domain_scores_codex":[0.9983055,0.0001680726,0.0004779098,0.0002439744,0.0004759227,0.0003285974],"domain_scores_gemma":[0.9985757,0.00002731053,0.000291826,0.0002536143,0.0005628425,0.0002886799],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001063326,0.0002137125,0.0006640227,0.000008515594,0.001235824,0.000008066106,0.0002291717,0.0002842476,0.989525,0.00003572959,0.006324797,0.0004076158],"study_design_scores_gemma":[0.003601128,0.001492903,0.0004218103,0.00001822497,0.0002659312,0.000020893,0.000213969,0.0009785007,0.9763538,0.0001464009,0.01623811,0.0002483214],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9848149,0.001781067,0.011843,0.0001059953,0.0002794639,0.0002165802,0.00002708369,0.000006798058,0.0009250983],"genre_scores_gemma":[0.9785299,0.0002569025,0.01814835,0.0001326555,0.0007877809,0.00001945485,0.0001013171,0.00004083138,0.001982753],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01317117,"threshold_uncertainty_score":0.5890873,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04917753157794701,"score_gpt":0.4418327896324559,"score_spread":0.3926552580545089,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}