{"id":"W41560684","doi":"10.2144/00281st04","title":"Localized Electroporation: A Method for Targeting Expression of Genes in Avian Embryos","year":2000,"lang":"en","type":"article","venue":"BioTechniques","topic":"Microbial Inactivation Methods","field":"Biochemistry, Genetics and Molecular Biology","cited_by":29,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Victoria","funders":"University of Georgia Research Foundation; China Scholarship Council; University of Victoria; University of Georgia","keywords":"Electroporation; Green fluorescent protein; Biology; Cell biology; Transfection; Embryo; Mesenchyme; Neural crest; Molecular biology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005167475,0.00009511386,0.0001420289,0.00005386266,0.0000295745,0.000007170129,0.0001027769,0.0002190202,0.00007427763],"category_scores_gemma":[0.00008639324,0.00009012367,0.00006046259,0.0001028629,0.00002516099,0.000004707218,0.00001964285,0.00005301919,4.503065e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001450841,"about_ca_system_score_gemma":0.00003587009,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006147089,"about_ca_topic_score_gemma":0.000009111558,"domain_scores_codex":[0.9991739,0.0001720551,0.0002551322,0.0002217272,0.00004891762,0.0001282808],"domain_scores_gemma":[0.9996333,0.00001713504,0.00008901008,0.0001720056,0.00006754464,0.00002107787],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002064554,0.00003136126,0.00003939596,0.00002140471,0.000003371528,1.734685e-7,0.00002682879,0.00001360735,0.9749592,0.00005222755,0.0013417,0.02330426],"study_design_scores_gemma":[0.0002026622,0.0001842081,0.00003337337,0.00001773611,0.000002993741,0.000001965834,0.00001057778,0.0001193931,0.9390203,0.0002637911,0.06004483,0.00009812164],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1835426,0.0004180549,0.8152037,0.00009545926,0.00001346504,0.0004326051,0.00001048168,0.00002981458,0.0002538576],"genre_scores_gemma":[0.1446977,0.0002498665,0.854406,0.0001173719,0.00008960635,0.00007354382,0.0001205075,0.00001860462,0.0002267566],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.05870313,"threshold_uncertainty_score":0.3675137,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01388932096639079,"score_gpt":0.3356074422667985,"score_spread":0.3217181213004077,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}