{"id":"W4200182084","doi":"10.22541/au.163687913.34133715/v1","title":"ONLY A SMALL FRACTION OF CELLS PRODUCE ASSEMBLED CAPSIDS DURING TRANSFECTION-BASED MANUFACTURING OF ADENO-ASSOCIATED VIRUS VECTORS","year":2021,"lang":"en","type":"preprint","venue":"","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; McGill University","funders":"","keywords":"Transfection; Capsid; Adeno-associated virus; Flow cytometry; Plasmid; Vector (molecular biology); Titer; Virus; HEK 293 cells; Viral vector; Molecular biology; Virology; Cell biology; Recombinant DNA; Chemistry; Biology; Cell culture; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009922656,0.0005337549,0.0009569459,0.0003862182,0.0003592926,0.001416736,0.0002768658,0.0004422229,0.001694981],"category_scores_gemma":[0.00106462,0.0003147691,0.0004058289,0.0004263554,0.0005294699,0.0005719695,0.000451999,0.0007570404,0.002483087],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004340691,"about_ca_system_score_gemma":0.0003636641,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006592947,"about_ca_topic_score_gemma":0.0006768616,"domain_scores_codex":[0.9991363,0.0001357187,0.00008210818,0.0002312017,0.0003153138,0.00009946752],"domain_scores_gemma":[0.9993286,0.0003055954,0.00008851988,0.0001179372,0.0001218906,0.00003745059],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004818656,0.00001723534,0.0006971057,0.00004498932,0.000007261032,0.00004719572,0.00007795981,0.0001805803,0.9951321,0.0002352001,0.00007461756,0.003437426],"study_design_scores_gemma":[0.00000514908,0.00008878288,0.002215733,0.000007042079,0.0000113311,0.0001534658,0.00003274283,0.001134986,0.9920774,0.0001620848,0.00410505,0.000006146483],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.758786,0.002884083,0.2272592,0.0002543779,0.0001480361,0.0003820717,0.001443332,0.0009744541,0.00786848],"genre_scores_gemma":[0.9172616,0.002261703,0.06684696,0.0003106413,0.000048392,0.0003512582,0.003230958,0.0004480398,0.009240294],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001694981,"threshold_uncertainty_score":0.005670249,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01674557584332158,"score_gpt":0.2694862727420926,"score_spread":0.252740696898771,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}