{"id":"W4200421804","doi":"10.2196/33746","title":"Multiplex Droplet Digital Polymerase Chain Reaction Assay for Rapid Molecular Detection of Pathogens in Patients With Sepsis: Protocol for an Assay Development Study","year":2021,"lang":"en","type":"article","venue":"JMIR Research Protocols","topic":"Bacterial Identification and Susceptibility Testing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"","keywords":"Polymerase chain reaction; Digital polymerase chain reaction; Protocol (science); Multiplex polymerase chain reaction; Multiplex; Sepsis; Computational biology; Medicine; Biology; Microbiology; Bioinformatics; Immunology; Gene; Genetics; Pathology","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001793704,0.0001575594,0.0001862409,0.0001265616,0.0001489419,0.000127858,0.0001628974,0.0001298695,0.000006066181],"category_scores_gemma":[0.0009879097,0.000146281,0.00005881725,0.0002630462,0.00004889598,0.00003239411,0.00009450866,0.0001293816,9.052172e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007570096,"about_ca_system_score_gemma":0.0003438543,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009697193,"about_ca_topic_score_gemma":0.0001779553,"domain_scores_codex":[0.9976165,0.0005782575,0.0004619634,0.000612661,0.0003805368,0.0003500645],"domain_scores_gemma":[0.9980455,0.00006484696,0.000158173,0.0004835848,0.001144323,0.0001035826],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.002566223,0.00418744,0.02723308,0.0002950539,0.00002986246,0.000001109712,0.0001902858,0.000003005548,0.9309863,0.000001965366,0.00002598148,0.03447974],"study_design_scores_gemma":[0.009515884,0.006662058,0.09005386,0.0002354376,0.000002583347,8.100597e-7,0.0005130309,0.0001390784,0.8784183,0.00001439198,0.01417806,0.0002665427],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"protocol","genre_gemma":"protocol","genre_scores_codex":[0.3683057,1.806694e-7,0.001674601,0.00001043038,0.000003705988,0.6299435,0.00003873053,0.00001076193,0.00001244533],"genre_scores_gemma":[0.3810684,7.092265e-9,0.0006911351,0.000005149815,0.00002636007,0.6179006,0.0002338226,0.00002298602,0.00005158518],"genre_candidate":"protocol","genre_consensus":"protocol","teacher_disagreement_score":0.06282079,"threshold_uncertainty_score":0.5965167,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.122128216640976,"score_gpt":0.4519420394486979,"score_spread":0.3298138228077219,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}