{"id":"W4200592086","doi":"10.3389/fcell.2021.789959","title":"Mitochondria Endoplasmic Reticulum Contact Sites (MERCs): Proximity Ligation Assay as a Tool to Study Organelle Interaction","year":2021,"lang":"en","type":"review","venue":"Frontiers in Cell and Developmental Biology","topic":"Mitochondrial Function and Pathology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":32,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université du Québec à Trois-Rivières; Université du Québec à Montréal","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Endoplasmic reticulum; Organelle; Proximity ligation assay; Mitochondrion; Cell biology; Membrane contact site; Biology; Endogeny; Calcium signaling; Förster resonance energy transfer; STIM1; Computational biology; Biophysics; Fluorescence; Biochemistry; Signal transduction; Membrane protein; Membrane; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0003294518,0.0004898602,0.00110824,0.0002551575,0.0001173167,0.00004376528,0.0002032527,0.0006921111,0.0001883097],"category_scores_gemma":[0.0002769824,0.000441217,0.0001586501,0.0002841247,0.00005298783,0.00001176066,0.0003210115,0.0003715051,0.00008987889],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001054311,"about_ca_system_score_gemma":0.0003285268,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002848295,"about_ca_topic_score_gemma":0.00006990699,"domain_scores_codex":[0.9970598,0.0006932684,0.0007165247,0.001073006,0.00009160913,0.0003658281],"domain_scores_gemma":[0.9992484,0.00005945512,0.0002244938,0.0002741941,0.00006665417,0.000126807],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0007666888,0.001634879,0.01406256,0.002185273,0.001067699,0.0002608753,0.001647995,0.000001739631,0.1178858,0.00006463017,0.06606414,0.7943577],"study_design_scores_gemma":[0.0008962401,0.0008702612,0.00004926218,0.000319913,0.0001395325,0.0002480072,0.001190632,0.000001628014,0.009512112,0.00003285691,0.9860976,0.0006420045],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.06645018,0.9201556,0.002327573,0.00003968368,0.005762812,0.003201389,0.00007861629,0.00003467031,0.001949469],"genre_scores_gemma":[0.002859926,0.9736196,0.01677175,0.000420564,0.000375523,0.0005616277,0.00302425,0.000069196,0.00229756],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.9200334,"threshold_uncertainty_score":0.999804,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01751777001510498,"score_gpt":0.2806612193479994,"score_spread":0.2631434493328944,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}