{"id":"W4205305860","doi":"10.1111/febs.16354","title":"Chimeric mutants of staphylococcal hemolysin, which act as both one‐component and two‐component hemolysin, created by grafting the stem domain","year":2022,"lang":"en","type":"article","venue":"FEBS Journal","topic":"Nanopore and Nanochannel Transport Studies","field":"Engineering","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Aging","funders":"Japan Society for the Promotion of Science; New Energy and Industrial Technology Development Organization; Japan Agency for Medical Research and Development","keywords":"Hemolysin; Mutant; Transmembrane protein; Biophysics; Pore-forming toxin; Protein engineering; Chemistry; Biology; Cell biology; Biochemistry; Microbial toxins; Receptor; Virulence; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001577613,0.0004655644,0.0002477303,0.0001653358,0.0001040081,0.0001604746,0.0003317306,0.0002879993,0.0005213087],"category_scores_gemma":[0.0001360755,0.0001193316,0.0003386963,0.0001883485,0.0002141812,0.000184866,0.0002196476,0.0005632486,0.0001685325],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003583528,"about_ca_system_score_gemma":0.0002176685,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007209383,"about_ca_topic_score_gemma":0.000673435,"domain_scores_codex":[0.9998497,0.00002953757,0.00001812293,0.00003200877,0.00004421013,0.00002639718],"domain_scores_gemma":[0.9998158,0.00003126194,0.00007160578,0.00002230479,0.00001700383,0.00004207998],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006500015,0.00002975983,0.00009613805,0.00001816247,0.000007591642,0.00005360752,0.00001253422,0.00005752497,0.9992155,0.00009790032,0.00001336996,0.0003327974],"study_design_scores_gemma":[0.00002093448,0.000261245,0.002113326,0.000004182598,0.00002806581,0.0003414183,0.00002647649,0.001154071,0.9941556,0.00003591206,0.00185192,0.000006751037],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.99419,0.0003268155,0.004626142,0.00003509051,0.00002346475,0.00004933147,0.0002866792,0.00004121389,0.0004211853],"genre_scores_gemma":[0.9912934,0.0002880584,0.005853756,0.00004019003,0.00000634866,0.00005720878,0.001012648,0.00003192854,0.001416538],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007209383,"threshold_uncertainty_score":0.002600074,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009339646164359493,"score_gpt":0.2074063087730158,"score_spread":0.1980666626086563,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}