{"id":"W4205305860","doi":"10.1111/febs.16354","title":"Chimeric mutants of staphylococcal hemolysin, which act as both one‐component and two‐component hemolysin, created by grafting the stem domain","year":2022,"lang":"en","type":"article","venue":"FEBS Journal","topic":"Nanopore and Nanochannel Transport Studies","field":"Engineering","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Aging","funders":"Japan Society for the Promotion of Science; New Energy and Industrial Technology Development Organization; Japan Agency for Medical Research and Development","keywords":"Hemolysin; Mutant; Transmembrane protein; Biophysics; Pore-forming toxin; Protein engineering; Chemistry; Biology; Cell biology; Biochemistry; Microbial toxins; Receptor; Virulence; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0006810962,0.0003262122,0.000550136,0.0001800377,0.0008715377,0.00006022628,0.0003240656,0.00004631959,0.0001792177],"category_scores_gemma":[0.000006745744,0.0002600266,0.0001607723,0.0006205734,0.00008250657,0.0001197359,0.0001235975,0.0007376133,0.000003883984],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000121473,"about_ca_system_score_gemma":0.00004583883,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007345565,"about_ca_topic_score_gemma":0.00001376261,"domain_scores_codex":[0.9977361,0.000155691,0.000681965,0.00024872,0.0006514505,0.0005260779],"domain_scores_gemma":[0.999184,0.0001028296,0.0002325392,0.0002353308,0.00007900692,0.0001663136],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006050204,0.000777177,0.01478706,0.0002228076,0.002989393,0.0003948245,0.008097898,0.02178615,0.9354914,0.0001497868,0.004684014,0.01001441],"study_design_scores_gemma":[0.035723,0.004507612,0.1172282,0.001569287,0.003267198,0.01101744,0.03461194,0.02558693,0.6776775,0.004897296,0.07660048,0.007313099],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9911751,0.006619805,0.000638807,0.0003307107,0.0003984778,0.0002600111,0.00007031051,0.00008671336,0.0004201299],"genre_scores_gemma":[0.9986372,0.0007947484,0.0001948531,0.00005909734,0.0001318972,0.0000235097,0.00001924267,0.00005923025,0.0000802424],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.257814,"threshold_uncertainty_score":0.9999852,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009339646164359493,"score_gpt":0.2074063087730158,"score_spread":0.1980666626086563,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}