{"id":"W42055240","doi":"10.2144/03352bm05","title":"<i>Escherichia coli endA</i> deletion strain for use in two-hybrid shuttle vector selection","year":2003,"lang":"en","type":"article","venue":"BioTechniques","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Manitoba","funders":"","keywords":"Shuttle vector; Saccharomyces cerevisiae; Genetics; Strain (injury); Library science; Biology; Gene; Vector (molecular biology); Computer science; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005495224,0.00217452,0.001630579,0.001805096,0.001032513,0.000694846,0.001871734,0.0008362636,0.0160362],"category_scores_gemma":[0.0006974423,0.001321273,0.001076168,0.001528308,0.0005339862,0.000561891,0.0007291075,0.003219266,0.01419189],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005709967,"about_ca_system_score_gemma":0.0007449233,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002423868,"about_ca_topic_score_gemma":0.005506469,"domain_scores_codex":[0.999121,0.0001826524,0.0001018417,0.0001650599,0.0002530529,0.0001762995],"domain_scores_gemma":[0.9989977,0.000263662,0.0001173342,0.0002648917,0.0001751208,0.00018134],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002583548,0.0001555192,0.0001746653,0.0001394641,0.000020543,0.00009172947,0.00003633381,0.00007958658,0.9926669,0.0006765981,0.002856929,0.002843325],"study_design_scores_gemma":[0.00007472759,0.0001439035,0.002625495,0.0000323804,0.00007509802,0.000524471,0.00003132567,0.0007036439,0.9563897,0.0002738181,0.03908044,0.00004508049],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3278848,0.00207779,0.4487903,0.00338899,0.002207513,0.004713413,0.1355937,0.02175266,0.05359079],"genre_scores_gemma":[0.2799537,0.002665383,0.2840943,0.001610003,0.0002860281,0.0025676,0.3096454,0.0108043,0.1083733],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.0160362,"threshold_uncertainty_score":0.05364645,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01410319644507479,"score_gpt":0.2618385994226249,"score_spread":0.2477354029775501,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}