{"id":"W42055240","doi":"10.2144/03352bm05","title":"<i>Escherichia coli endA</i> deletion strain for use in two-hybrid shuttle vector selection","year":2003,"lang":"en","type":"article","venue":"BioTechniques","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Manitoba","funders":"","keywords":"Shuttle vector; Saccharomyces cerevisiae; Genetics; Strain (injury); Library science; Biology; Gene; Vector (molecular biology); Computer science; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002540069,0.0001770541,0.0001552539,0.00007982281,0.00005653389,0.00004064544,0.0001182195,0.0003124468,0.0000142148],"category_scores_gemma":[0.0001096167,0.0001821794,0.00007741529,0.0001353868,0.00006191969,0.000005870671,0.00003341799,0.0001270951,0.000001904003],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003408318,"about_ca_system_score_gemma":0.00007353414,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008079033,"about_ca_topic_score_gemma":0.0002543999,"domain_scores_codex":[0.9989471,0.0000801531,0.0002272675,0.0003916275,0.00005972217,0.0002940909],"domain_scores_gemma":[0.9995723,0.00001093594,0.00007912152,0.0002202898,0.00007179005,0.00004554451],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007502516,0.00006600003,0.0003027261,0.000009896124,0.0000114928,7.491734e-7,0.000002966901,0.000003969725,0.994482,0.001329772,0.001112616,0.002602832],"study_design_scores_gemma":[0.0003594099,0.0005226657,0.0003855859,0.000008622921,0.000007091879,0.000009089047,0.00000462524,0.00005319122,0.8341136,0.0003660639,0.163978,0.0001920523],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9587378,0.0001482767,0.03981354,0.000132987,0.000172358,0.0006480991,0.0001173364,0.00008898746,0.0001406526],"genre_scores_gemma":[0.9553779,0.0002249075,0.04361407,0.0002103358,0.0001297302,0.000124553,0.000170299,0.0000322338,0.0001159647],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1628654,"threshold_uncertainty_score":0.7429059,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01410319644507479,"score_gpt":0.2618385994226249,"score_spread":0.2477354029775501,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}