{"id":"W4205957954","doi":"10.3791/51455-v","title":"Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale","year":2014,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":58,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University Health Centre; McGill University","funders":"Canadian Institutes of Health Research; Vetenskapsrådet; Cancerfonden","keywords":"Ribosome; Translation (biology); Polysome; Ribosome profiling; Biology; Protein biosynthesis; Cell biology; Messenger RNA; Computational biology; RNA; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003474342,0.0006579784,0.0004555408,0.001913243,0.0007785117,0.0005263537,0.0003490941,0.0003670769,0.001478546],"category_scores_gemma":[0.0003288074,0.0001970134,0.0005163916,0.001416265,0.0003402848,0.0002274793,0.0004612507,0.0008215331,0.000963702],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004262266,"about_ca_system_score_gemma":0.0004192479,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002025287,"about_ca_topic_score_gemma":0.004877232,"domain_scores_codex":[0.999716,0.00002194926,0.00002200537,0.0001065746,0.0001009578,0.00003252487],"domain_scores_gemma":[0.9998481,0.00003280869,0.00002848528,0.00002507688,0.00004467351,0.0000207849],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000600154,0.000009262276,0.0003280045,0.00007792291,0.00001590212,0.00004462101,0.00003953007,0.00009042959,0.9951906,0.0002048739,0.0001300143,0.003808733],"study_design_scores_gemma":[0.00002039363,0.0002063165,0.04159153,0.00003584071,0.0001129955,0.0004476098,0.0001128506,0.005283179,0.9287868,0.0006096515,0.02275838,0.00003444967],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6218139,0.009562169,0.3197036,0.0005348214,0.0005111002,0.0009160302,0.03389634,0.002825878,0.01023614],"genre_scores_gemma":[0.6114886,0.0121425,0.3092282,0.0006542341,0.0002258163,0.002144186,0.04635648,0.001659229,0.01610077],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002025287,"threshold_uncertainty_score":0.004946232,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01408702061327305,"score_gpt":0.352066852761044,"score_spread":0.3379798321477709,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}