{"id":"W4205968583","doi":"10.3791/3288-v","title":"Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips","year":2012,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Neuroscience and Neural Engineering","field":"Neuroscience","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; Hotchkiss Brain Institute; Institute for Biological Sciences; Institute for Microstructural Sciences","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Alberta Heritage Foundation for Medical Research; Fondation pour la Recherche Médicale","keywords":"Pipette; Patch clamp; Materials science; Microfluidics; Nanotechnology; Micromanipulator; Aperture (computer memory); Electrophysiology; Microelectrode; Biomedical engineering; Inverted microscope; Polydimethylsiloxane; Ion channel; Optoelectronics; Electrode; Computer science; Chemistry; Microscope; Optics; Engineering","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002489503,0.0008864973,0.002211974,0.000854965,0.0009057265,0.001386424,0.003630973,0.001272642,0.008755956],"category_scores_gemma":[0.002042883,0.0006256534,0.0009865284,0.0009362343,0.0008013842,0.0007387361,0.001000229,0.002141842,0.0153753],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006716708,"about_ca_system_score_gemma":0.0006371235,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00132954,"about_ca_topic_score_gemma":0.003397284,"domain_scores_codex":[0.9961399,0.0003715854,0.0006651361,0.000984503,0.001547349,0.0002915711],"domain_scores_gemma":[0.9976583,0.0004577825,0.0001790936,0.0009789192,0.0005640315,0.0001618145],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001080927,0.00006727303,0.0003757427,0.0002604295,0.00002026534,0.0001631515,0.0001453325,0.0005319862,0.9891506,0.0006883029,0.002767069,0.005721672],"study_design_scores_gemma":[0.00008102774,0.0006374523,0.006598726,0.0001034554,0.0001104471,0.0008153757,0.00009090215,0.01226549,0.9181,0.0006414656,0.06042736,0.0001283796],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1456145,0.004088125,0.7737499,0.001152237,0.001760395,0.007197781,0.03058643,0.01747059,0.01837999],"genre_scores_gemma":[0.1525014,0.003622246,0.7993233,0.001129251,0.0002197675,0.009514856,0.01863638,0.001482653,0.01357016],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.008755956,"threshold_uncertainty_score":0.02929157,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04955685987783372,"score_gpt":0.4025665304965861,"score_spread":0.3530096706187524,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}