{"id":"W4205981044","doi":"10.21203/rs.3.rs-704571/v1","title":"Kinetic, Catalytic and Thermodynamic Properties of Immobilized Milk Clotting Enzyme on Activated Chitosan Polymer and its Application in Cheese Making","year":2021,"lang":"en","type":"preprint","venue":"Research Square","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"National Research Council Canada","funders":"","keywords":"Chemistry; Enthalpy; Gibbs free energy; Glutaraldehyde; Bacillus circulans; Catalysis; Immobilized enzyme; Ionic strength; Reaction rate constant; Equilibrium constant; Chitosan; Covalent bond; Michaelis–Menten kinetics; Polymer; Activation energy; Turnover number; Polymer chemistry; Chromatography; Chemical engineering; Physical chemistry; Kinetics; Organic chemistry; Thermodynamics; Enzyme assay; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002800619,0.0002945451,0.0002077282,0.0003183314,0.000132679,0.0003137232,0.0003407238,0.0003213462,0.001066346],"category_scores_gemma":[0.0005793645,0.0001506287,0.0004112743,0.0003549933,0.0002633432,0.0001916247,0.0001025232,0.0003818857,0.0001777473],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003322566,"about_ca_system_score_gemma":0.0001965039,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002255527,"about_ca_topic_score_gemma":0.001241824,"domain_scores_codex":[0.9998562,0.00001962323,0.000008373439,0.0000296973,0.00005251894,0.00003346161],"domain_scores_gemma":[0.9998431,0.00006237381,0.00002627793,0.00001237472,0.00003660024,0.00001926824],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004323581,0.00003138503,0.0004595456,0.00007215578,0.00001513818,0.00006603565,0.00002306163,0.0006797879,0.9951453,0.000141798,0.00005633025,0.002877032],"study_design_scores_gemma":[0.000007741461,0.0001350028,0.003132293,0.000003350463,0.0000170188,0.00004275251,0.000009780432,0.003132303,0.9931086,0.00003311077,0.0003659218,0.00001209866],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9935321,0.002123813,0.0022696,0.0000639147,0.00002498891,0.00001295965,0.0001963218,0.00002848909,0.001747856],"genre_scores_gemma":[0.9973512,0.0004873612,0.0007471684,0.000009163198,0.000008798675,0.000005646203,0.000125365,0.000008413299,0.00125692],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002255527,"threshold_uncertainty_score":0.004484713,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02987452139777678,"score_gpt":0.3247367657546878,"score_spread":0.294862244356911,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}