{"id":"W4206065046","doi":"10.3791/2160-v","title":"Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts","year":2010,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Cre recombinase; Transduction (biophysics); Biology; Recombinase; Cre-Lox recombination; Molecular biology; Embryonic stem cell; Cell biology; Transgene; 3T3 cells; Gene; RAC1; Signal transduction; Transfection; Genetically modified mouse; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001145318,0.0009327662,0.0008323324,0.0007179757,0.0004664895,0.0006305889,0.001228616,0.0007670033,0.003867671],"category_scores_gemma":[0.0003272706,0.0005767651,0.0006895402,0.0005405034,0.0003747397,0.0003581163,0.0005456756,0.00198785,0.004213273],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005222918,"about_ca_system_score_gemma":0.0005861561,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001035274,"about_ca_topic_score_gemma":0.001986826,"domain_scores_codex":[0.9990393,0.00009376462,0.0001832488,0.0002034125,0.0003677691,0.0001125508],"domain_scores_gemma":[0.9995953,0.00007992422,0.00006863862,0.0001155268,0.00006572638,0.00007486121],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006293413,0.00004513796,0.00008855284,0.0001207153,0.000006474584,0.00008885026,0.00004359453,0.00006897554,0.9969681,0.0003588155,0.0002332002,0.001914721],"study_design_scores_gemma":[0.00004870796,0.0003485947,0.001539604,0.00003865292,0.00003869463,0.0004303602,0.00003366734,0.001075564,0.9654577,0.0001348888,0.03083678,0.00001689444],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6494925,0.01870832,0.2592679,0.000633575,0.002641272,0.003777196,0.03277744,0.004498289,0.02820352],"genre_scores_gemma":[0.6766136,0.01615893,0.216578,0.000500057,0.000245712,0.004413071,0.02727419,0.0006063548,0.05761008],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.003867671,"threshold_uncertainty_score":0.01293868,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01029835342913329,"score_gpt":0.342958491501324,"score_spread":0.3326601380721907,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}