{"id":"W4206332693","doi":"10.3791/50031-v","title":"Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)","year":2013,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Palmitoylation; Biotinylation; Biotin; Cysteine; Immunoprecipitation; Biochemistry; Chemistry; Thioester; Streptavidin; Lipid-anchored protein; Autophagy; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008466496,0.002276094,0.00192629,0.001404769,0.001000814,0.0009600876,0.001324467,0.0008413398,0.002053131],"category_scores_gemma":[0.0004211407,0.0009530836,0.001152441,0.001247248,0.0006885382,0.0008098276,0.00140466,0.002624666,0.005441151],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008529832,"about_ca_system_score_gemma":0.0006692488,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002286152,"about_ca_topic_score_gemma":0.007254136,"domain_scores_codex":[0.9985726,0.000091083,0.00016909,0.0004049714,0.0004763859,0.0002859026],"domain_scores_gemma":[0.9995782,0.00007135899,0.00006854038,0.00006928828,0.0001560516,0.00005651479],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004610377,0.00002822522,0.0001227886,0.0001188317,0.00001518068,0.00005072605,0.00002878005,0.00002652809,0.9979418,0.00007488464,0.00009166373,0.001454292],"study_design_scores_gemma":[0.00001734634,0.0001272214,0.003360439,0.00003044982,0.00005184369,0.0002893617,0.00004961166,0.001034173,0.9894595,0.0001405143,0.005422294,0.00001719022],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3866768,0.0358295,0.5232562,0.001439398,0.002221565,0.002577762,0.02054502,0.005112624,0.02234112],"genre_scores_gemma":[0.3707405,0.03355224,0.540259,0.001402758,0.0005282521,0.005159684,0.02052044,0.001148754,0.02668829],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002286152,"threshold_uncertainty_score":0.006868422,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009829810730562208,"score_gpt":0.3316834977003623,"score_spread":0.3218536869698001,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}