{"id":"W4206334536","doi":"10.3791/3422-v","title":"Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization","year":2012,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Cell death mechanisms and regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Canadian Institutes of Health Research; Hospital for Sick Children; Multiple Sclerosis Society; Natural Sciences and Engineering Research Council of Canada; Multiple Sclerosis Society of Canada","keywords":"Caspase; Cell biology; Programmed cell death; Biology; Oligodendrocyte; Depolarization; Neuroscience; Apoptosis; Neurodegeneration; Population; Myelin; Central nervous system; Pathology; Biochemistry; Medicine; Endocrinology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005434381,0.0005144346,0.0004271285,0.0006706601,0.0003159727,0.0004714329,0.0005807409,0.0004207007,0.001144283],"category_scores_gemma":[0.0002312221,0.0001979308,0.0003253515,0.000703616,0.0003582638,0.000380795,0.0003822287,0.001097147,0.0005884119],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004558909,"about_ca_system_score_gemma":0.0002996829,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009082348,"about_ca_topic_score_gemma":0.001689587,"domain_scores_codex":[0.9995148,0.00005483552,0.00007784525,0.00009753484,0.0001775047,0.00007743017],"domain_scores_gemma":[0.9997795,0.00004162064,0.00006423055,0.00003271989,0.00004355222,0.00003841348],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004031078,0.00001973548,0.0001046932,0.00002758216,0.000002221414,0.00003075487,0.00002953987,0.00002418129,0.999166,0.00005802239,0.00001720817,0.0004797823],"study_design_scores_gemma":[0.000007951786,0.0001335502,0.002418491,0.000005576301,0.0000086524,0.0001971955,0.00002755171,0.0008002586,0.995263,0.00004409667,0.001087884,0.000005710706],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.906836,0.004481405,0.08146239,0.0001261165,0.0001208987,0.000342887,0.002451769,0.000594131,0.003584458],"genre_scores_gemma":[0.8292409,0.006188693,0.1504065,0.0001767949,0.0000488885,0.001109938,0.004563102,0.0001395231,0.008125521],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001144283,"threshold_uncertainty_score":0.003828049,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02273770853191916,"score_gpt":0.3446276010238445,"score_spread":0.3218898924919253,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}