{"id":"W4206547853","doi":"10.1016/j.xpro.2021.101108","title":"Immunoprecipitation and Western blot-based detection of protein O-GlcNAcylation in cells","year":2022,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University; Université de Montréal; Hôpital Maisonneuve-Rosemont","funders":"Fonds de Recherche du Québec - Santé; Canadian Institutes of Health Research","keywords":"Immunoprecipitation; Western blot; Blot; HEK 293 cells; Transfection; HeLa; Molecular biology; Cell biology; Chemistry; Protein detection; Biology; Cell; Gene; Biochemistry; Nanotechnology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003902334,0.00007464532,0.00008443196,0.0001129231,0.00007656572,0.00001363683,0.00008288216,0.00004799412,0.00004223542],"category_scores_gemma":[0.00003246231,0.00008173072,0.00002611833,0.0001562532,0.0000330647,0.000005906465,0.00007629127,0.0001066611,0.000001733841],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002979593,"about_ca_system_score_gemma":0.00007530158,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008848798,"about_ca_topic_score_gemma":0.0001146536,"domain_scores_codex":[0.9990738,0.0001918431,0.0002042949,0.0001993108,0.000207027,0.0001237457],"domain_scores_gemma":[0.9996327,0.000007287679,0.00009374548,0.0001766757,0.00006357238,0.00002604994],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004851827,0.00008855184,0.001443601,0.00005928461,0.000004055908,6.347684e-7,0.00007089643,0.0005089506,0.9912711,0.000007947428,0.000004446141,0.006055376],"study_design_scores_gemma":[0.001419094,0.001103279,0.007716912,0.00001974851,0.000001169465,9.954113e-7,0.00007671144,0.0009706777,0.9788812,0.00006197308,0.009655043,0.00009320729],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9680355,0.00002786748,0.001155151,0.00004500373,0.00001223188,0.0306226,0.00001771848,0.000006867635,0.00007709626],"genre_scores_gemma":[0.9443473,7.543783e-7,0.0004023577,0.00001853134,0.00001424489,0.05495111,0.00004120805,0.00001165796,0.0002128163],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0243285,"threshold_uncertainty_score":0.3332882,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01358828630029262,"score_gpt":0.291869803108202,"score_spread":0.2782815168079094,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}