{"id":"W4206986373","doi":"10.3389/fgene.2021.591543","title":"Fluoride-Controlled Riboswitch-Based Dampening of Gene Expression for Cloning Potent Promoters","year":2022,"lang":"en","type":"article","venue":"Frontiers in Genetics","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institut National de la Recherche Scientifique","funders":"Fonds de Recherche du Québec - Santé; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Armand-Frappier Foundation","keywords":"Bioreporter; Promoter; Reporter gene; Riboswitch; Biology; Multiple cloning site; Luciferase; Plasmid; Gene; Gene expression; Genetics; Computational biology; Expression vector; Recombinant DNA; Non-coding RNA; Transfection","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003007499,0.0005267311,0.0005299018,0.0002015142,0.0001704177,0.0004626347,0.0004339541,0.000766631,0.001089376],"category_scores_gemma":[0.0005938612,0.0002467918,0.0003888595,0.0001745705,0.0004522978,0.0005477502,0.0003714433,0.001079274,0.000509098],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004012956,"about_ca_system_score_gemma":0.0002139475,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003081294,"about_ca_topic_score_gemma":0.0005689884,"domain_scores_codex":[0.9996783,0.00004106107,0.00001937573,0.0001105716,0.000102074,0.00004857544],"domain_scores_gemma":[0.9996392,0.0001404173,0.00009493762,0.00004460232,0.00004152116,0.00003935492],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001584961,0.000006280152,0.00002617012,0.000031594,0.000001873757,0.00002531271,0.000008415498,0.00009193655,0.9985475,0.0001387529,0.00001562977,0.001090669],"study_design_scores_gemma":[0.000003746405,0.00006709911,0.0001467811,0.00000385376,0.000003659993,0.0000872381,0.000005999215,0.000905222,0.9973866,0.00007232478,0.001309738,0.000007618215],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7649024,0.001768226,0.226105,0.0004305919,0.0002030454,0.0002971468,0.000594769,0.001627172,0.004071658],"genre_scores_gemma":[0.9435253,0.000988138,0.0511731,0.0001703941,0.00002729632,0.0001575016,0.0004256743,0.0001899357,0.003342552],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001089376,"threshold_uncertainty_score":0.003644288,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006026119057541279,"score_gpt":0.2582736493339288,"score_spread":0.2522475302763875,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}