{"id":"W4207085747","doi":"10.3791/3932-v","title":"Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates","year":2012,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; St. Michael's Hospital","funders":"","keywords":"Guanine nucleotide exchange factor; RHOA; Cell biology; GTPase; Biology; GTPase-activating protein; Mutant; G protein; Gene; Signal transduction; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000943288,0.001905484,0.001861906,0.00140578,0.0009777471,0.0006292165,0.001596431,0.0009734718,0.003738427],"category_scores_gemma":[0.0004417498,0.0008697697,0.001792126,0.001869463,0.0005222636,0.0005605732,0.001088282,0.00349436,0.005489087],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008785282,"about_ca_system_score_gemma":0.0007418685,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001733161,"about_ca_topic_score_gemma":0.004027615,"domain_scores_codex":[0.9981749,0.0002884807,0.0002260755,0.0004092965,0.0005501869,0.0003510403],"domain_scores_gemma":[0.9995421,0.0001163153,0.00005978769,0.00009640943,0.0001214934,0.00006380386],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007786614,0.0001437721,0.0001298726,0.000242306,0.00005680571,0.00007427092,0.00002847436,0.000114833,0.9961627,0.0002093133,0.0007597483,0.002000102],"study_design_scores_gemma":[0.00006264509,0.0003934348,0.00325324,0.00005569364,0.00009919265,0.0003866635,0.0000366295,0.002976655,0.9725519,0.0002760644,0.01986223,0.00004563756],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.4793221,0.04116531,0.4171837,0.003682529,0.003132821,0.005480606,0.02365335,0.005941733,0.02043789],"genre_scores_gemma":[0.413009,0.02633768,0.4076071,0.001862635,0.0006140592,0.004500686,0.09986902,0.001036033,0.04516378],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.003738427,"threshold_uncertainty_score":0.01250631,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02869488271459325,"score_gpt":0.3707744601684837,"score_spread":0.3420795774538904,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}