{"id":"W4210478208","doi":"10.1002/cpz1.353","title":"Efficient Gamma‐Retroviral Transduction of Primary Human Skin Cells Using the EF‐c Peptide as a Transduction Enhancer","year":2022,"lang":"en","type":"article","venue":"Current Protocols","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"SickKids Foundation; University of Toronto; Hospital for Sick Children; Université Laval","funders":"","keywords":"Transduction (biophysics); Biology; Keratinocyte; Fibroblast; Cell biology; Ex vivo; Genetic enhancement; Signal transduction; Molecular biology; Dermal fibroblast; Stem cell; Flow cytometry; Cell culture; In vitro; Gene; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000661524,0.000180905,0.0001814646,0.00007957579,0.0003607415,0.00002390507,0.0004594536,0.00005622187,0.000309033],"category_scores_gemma":[0.000006624624,0.0001565453,0.0002039422,0.0002375993,0.000195108,0.000004449135,0.0001250471,0.0003121075,0.000004059746],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000125497,"about_ca_system_score_gemma":0.0002335208,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001685203,"about_ca_topic_score_gemma":0.000008728121,"domain_scores_codex":[0.9980212,0.0003993322,0.0003389766,0.0004174015,0.0005081707,0.0003148608],"domain_scores_gemma":[0.9991187,0.000007652945,0.0001512449,0.000577122,0.00008773065,0.000057538],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001558209,0.0003855783,0.00002755403,0.0001308416,0.00003153912,5.478548e-7,0.0002850039,0.004734434,0.9797061,0.0000150833,0.0001603693,0.01296476],"study_design_scores_gemma":[0.0009881997,0.003034728,0.00004925649,0.00003253561,0.00001832671,0.000008335595,0.0001115428,0.0003204071,0.9707205,0.00003373555,0.02450633,0.0001760614],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9687868,0.0003594584,0.001042398,0.00005089556,0.0001947128,0.02937414,0.00003941772,0.00001361421,0.0001385229],"genre_scores_gemma":[0.9589632,0.00001561205,0.00007822797,0.0000301761,0.0003624182,0.04023061,0.00007967465,0.00003899821,0.000201102],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02434596,"threshold_uncertainty_score":0.6383733,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0424844896185379,"score_gpt":0.3632991158153516,"score_spread":0.3208146261968137,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}