{"id":"W4210478208","doi":"10.1002/cpz1.353","title":"Efficient Gamma‐Retroviral Transduction of Primary Human Skin Cells Using the EF‐c Peptide as a Transduction Enhancer","year":2022,"lang":"en","type":"article","venue":"Current Protocols","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"SickKids Foundation; University of Toronto; Hospital for Sick Children; Université Laval","funders":"","keywords":"Transduction (biophysics); Biology; Keratinocyte; Fibroblast; Cell biology; Ex vivo; Genetic enhancement; Signal transduction; Molecular biology; Dermal fibroblast; Stem cell; Flow cytometry; Cell culture; In vitro; Gene; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008365341,0.0008209125,0.0003034489,0.0005504673,0.0003773434,0.0003492917,0.0005342395,0.000545305,0.004165904],"category_scores_gemma":[0.0003010899,0.0002886342,0.000266472,0.0003960512,0.000374148,0.0003587433,0.0003109355,0.001005969,0.00208305],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002934419,"about_ca_system_score_gemma":0.0004434202,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004801191,"about_ca_topic_score_gemma":0.0007899085,"domain_scores_codex":[0.9995244,0.00008852228,0.00007913661,0.0001025206,0.000147679,0.00005787528],"domain_scores_gemma":[0.9998047,0.00006362712,0.00002751925,0.00004901834,0.0000323512,0.00002286375],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003480907,0.00001831965,0.0000256619,0.00006624287,0.000001285856,0.00002876991,0.00002042615,0.00002992425,0.9976683,0.0001794858,0.0001227996,0.00180383],"study_design_scores_gemma":[0.00001488155,0.00009392386,0.0005770139,0.00001343222,0.000006408439,0.0001196855,0.00000849002,0.0002566584,0.9924143,0.00005444815,0.006436286,0.000004531294],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6792387,0.00584393,0.2704842,0.000496785,0.0005107887,0.005603171,0.01022538,0.002351033,0.02524596],"genre_scores_gemma":[0.6737642,0.006210719,0.2538685,0.0003243315,0.00008783862,0.005459319,0.02010286,0.0004758498,0.03970631],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.004165904,"threshold_uncertainty_score":0.0139364,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0424844896185379,"score_gpt":0.3632991158153516,"score_spread":0.3208146261968137,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}