{"id":"W4210515920","doi":"10.1007/978-1-0716-1859-2_17","title":"Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins: From Expression Screening for Soluble Constructs to Protein Quality Control","year":2022,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Institute of Cancer Research; Institut National Du Cancer; Centre National de la Recherche Scientifique; Ligue Contre le Cancer; Université de Strasbourg; Institut National de la Santé et de la Recherche Médicale; Agence Nationale de la Recherche; Association pour la Recherche sur le Cancer","keywords":"Heterologous; Recombinant DNA; Computational biology; Homologous recombination; Baculoviridae; Inclusion bodies; Vector (molecular biology); Heterologous expression; Biology; Protein engineering; Cell biology; Biotechnology; Biochemistry; Gene; Enzyme","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009753435,0.001110312,0.001356281,0.00161871,0.0008208577,0.00122607,0.001097831,0.0007634884,0.002613509],"category_scores_gemma":[0.000432556,0.000790523,0.0008850675,0.001419129,0.0005529043,0.0006246956,0.0007602937,0.003347477,0.004973621],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006830104,"about_ca_system_score_gemma":0.0006987606,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00125958,"about_ca_topic_score_gemma":0.001868826,"domain_scores_codex":[0.9991525,0.0001292582,0.00009730292,0.0001591982,0.0003866339,0.00007502541],"domain_scores_gemma":[0.9996846,0.00007450621,0.00005065265,0.00008571654,0.00005784262,0.00004671951],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000084245,0.00005070403,0.00009558835,0.0001550339,0.00001745195,0.00004828845,0.00003905208,0.00003877238,0.9927133,0.0006352452,0.0007407853,0.005381529],"study_design_scores_gemma":[0.00004974302,0.0002449832,0.001824407,0.00003571407,0.00007621443,0.0004704435,0.00002172579,0.0008858123,0.9540207,0.0004202141,0.04191559,0.00003456815],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3075033,0.02396138,0.6052631,0.002200458,0.001468376,0.003802014,0.02484103,0.01215212,0.01880821],"genre_scores_gemma":[0.2581294,0.01754368,0.596018,0.001013941,0.0003032119,0.002232407,0.08050252,0.002387078,0.0418698],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002613509,"threshold_uncertainty_score":0.008743107,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03418868661103282,"score_gpt":0.3687002363940505,"score_spread":0.3345115497830177,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}