{"id":"W4210586290","doi":"10.3390/life12020171","title":"Cell Cycle Stage and DNA Repair Pathway Influence CRISPR/Cas9 Gene Editing Efficiency in Porcine Embryos","year":2022,"lang":"en","type":"article","venue":"Life","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Zygote; Embryo; Biology; CRISPR; Genome editing; Blastocyst; Cas9; Somatic cell; Cell biology; Cell cycle; Somatic cell nuclear transfer; Embryonic stem cell; Embryogenesis; Genetics; Andrology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003354408,0.0002195967,0.0002455811,0.0002009321,0.0001159643,0.0003684654,0.000152079,0.0003453171,0.001169765],"category_scores_gemma":[0.000337704,0.0001498428,0.0002654016,0.00008463678,0.0001838873,0.0002432512,0.0001702713,0.0004639227,0.0002467132],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002126319,"about_ca_system_score_gemma":0.000211495,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008908886,"about_ca_topic_score_gemma":0.0009655213,"domain_scores_codex":[0.9996685,0.00006684971,0.00004078389,0.00008311603,0.00007008683,0.00007067811],"domain_scores_gemma":[0.9997506,0.0001130052,0.00005454315,0.00001645814,0.00002881591,0.00003651127],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002433224,0.00003414243,0.001045131,0.00005460932,0.000007786612,0.00004736945,0.00002828322,0.00008949597,0.9960252,0.00004202331,0.00003935178,0.002343274],"study_design_scores_gemma":[0.00001979725,0.0009753015,0.03535981,0.00001858835,0.00007543265,0.0002700997,0.0000620669,0.001564102,0.9594072,0.00005554867,0.002167982,0.00002415705],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9924595,0.00216448,0.002876219,0.00005289932,0.00003622976,0.0000329417,0.0002405505,0.00004139846,0.002095898],"genre_scores_gemma":[0.9944489,0.0008871873,0.001389376,0.00007080019,0.000008239753,0.00004051002,0.0002992631,0.00002631034,0.002829353],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001169765,"threshold_uncertainty_score":0.003913283,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004220010429751007,"score_gpt":0.2421068767955596,"score_spread":0.2378868663658086,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}