{"id":"W4210586290","doi":"10.3390/life12020171","title":"Cell Cycle Stage and DNA Repair Pathway Influence CRISPR/Cas9 Gene Editing Efficiency in Porcine Embryos","year":2022,"lang":"en","type":"article","venue":"Life","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Zygote; Embryo; Biology; CRISPR; Genome editing; Blastocyst; Cas9; Somatic cell; Cell biology; Cell cycle; Somatic cell nuclear transfer; Embryonic stem cell; Embryogenesis; Genetics; Andrology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002257159,0.0001196066,0.0001141813,0.00005010281,0.0001005682,0.00001174886,0.0001190198,0.00004787818,0.00003157419],"category_scores_gemma":[0.00008361503,0.0001337142,0.0000419921,0.0001192881,0.00002387593,0.000003037844,0.0002561572,0.0001321922,0.000001876984],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001239837,"about_ca_system_score_gemma":0.00004717157,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004617459,"about_ca_topic_score_gemma":0.00001056984,"domain_scores_codex":[0.9990625,0.00003640114,0.0002022163,0.0003237148,0.0001290147,0.0002460963],"domain_scores_gemma":[0.9995869,0.00001091824,0.00004563968,0.0002479812,0.00001873229,0.0000898229],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000194453,0.00006142556,0.008721062,0.00004968126,0.000006702729,0.00003056182,0.0002200158,0.03853898,0.9516935,0.00001763853,0.0004700689,0.0001709484],"study_design_scores_gemma":[0.00143939,0.000529731,0.03384552,0.00001432041,0.00001301551,0.00003602905,0.00119416,0.009575668,0.9201455,0.00001333304,0.03267263,0.0005206609],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9959168,0.002203048,0.00099029,0.00005974318,0.0001436425,0.0001293332,0.00003110249,0.00002837219,0.0004976341],"genre_scores_gemma":[0.9985245,0.00005170274,0.0004084747,0.0003338869,0.0001618206,0.00003094786,0.00003096135,0.00001928312,0.0004384605],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03220256,"threshold_uncertainty_score":0.5452708,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004220010429751007,"score_gpt":0.2421068767955596,"score_spread":0.2378868663658086,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}