{"id":"W4210721558","doi":"10.3791/62950-v","title":"Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases","year":2021,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"","keywords":"Phage display; Phagemid; Ubiquitin; Bacteriophage; Filamentous bacteriophage; Biology; Ubiquitin ligase; Peptide library; Molecular biology; Computational biology; Biochemistry; Chemistry; Cell biology; Peptide sequence; Peptide; Escherichia coli; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004067844,0.0005049485,0.00045393,0.0003395552,0.0001689048,0.0006357394,0.0004057983,0.0005532317,0.0009512251],"category_scores_gemma":[0.0002749866,0.0002569508,0.0003518939,0.0003453373,0.0001865844,0.0003170635,0.0003861876,0.0007200118,0.0004961814],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005822043,"about_ca_system_score_gemma":0.0001606591,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003202449,"about_ca_topic_score_gemma":0.0004878383,"domain_scores_codex":[0.9997242,0.00004583937,0.00002248466,0.00006083709,0.00009469585,0.00005186619],"domain_scores_gemma":[0.9999208,0.00001427346,0.00001610437,0.000008082879,0.00001793924,0.00002283411],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001325941,0.0001115418,0.0002428243,0.00007875927,0.00001670354,0.0001350951,0.00003549234,0.0007173959,0.9904607,0.0006655674,0.0002728611,0.007130483],"study_design_scores_gemma":[0.00004438626,0.0004883141,0.0005060355,0.000009172953,0.00002494837,0.0002125777,0.00001481317,0.00354842,0.9803792,0.0001217277,0.01463829,0.00001212799],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8984258,0.01018395,0.07849047,0.0005907745,0.0003383397,0.0006850831,0.0007359011,0.001397449,0.009152247],"genre_scores_gemma":[0.9516432,0.004374912,0.03393249,0.0003818448,0.00005328259,0.0003936683,0.000775045,0.0001333839,0.008312229],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009512251,"threshold_uncertainty_score":0.004224181,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06411339611076809,"score_gpt":0.4746517402704022,"score_spread":0.4105383441596341,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}