{"id":"W4210894772","doi":"10.1002/anie.202112931","title":"A Kinetic and Fluorogenic Enhancement Strategy for Labeling of Nucleic Acids","year":2022,"lang":"en","type":"article","venue":"Angewandte Chemie International Edition","topic":"Click Chemistry and Applications","field":"Chemistry","cited_by":45,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Bioorthogonal chemistry; Tetrazine; Nucleic acid; Acridine; Intercalation (chemistry); Chemistry; DNA; Quenching (fluorescence); Combinatorial chemistry; Uridine; Conjugate; RNA; Molecular beacon; Fluorescence; Biochemistry; Click chemistry; Oligonucleotide; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005809228,0.0005504107,0.0003679609,0.0002804119,0.0001386942,0.0004617574,0.0005914543,0.0005549645,0.0008887037],"category_scores_gemma":[0.0004170298,0.0004069073,0.0002819851,0.0002004358,0.0003547457,0.0005043742,0.0004662919,0.0009590987,0.0004792716],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005523586,"about_ca_system_score_gemma":0.0003731245,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003706243,"about_ca_topic_score_gemma":0.0005263346,"domain_scores_codex":[0.9995223,0.00007812383,0.0000293508,0.0001437326,0.0001584208,0.00006802797],"domain_scores_gemma":[0.9997789,0.00004839462,0.00004780203,0.00004073631,0.00004104399,0.0000431988],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002113237,0.00001615592,0.00003125295,0.00002436762,0.000003081868,0.00001922125,0.00001015553,0.00009557194,0.9970986,0.000526531,0.00002910923,0.002124823],"study_design_scores_gemma":[0.000002118968,0.00003341481,0.00008545165,9.732842e-7,0.000003463598,0.00006561178,0.000001681035,0.0006132175,0.9981285,0.00002764556,0.001033606,0.000004403268],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7146833,0.004175992,0.2727104,0.0003622213,0.0002102122,0.0002052234,0.0002648392,0.0007631957,0.006624711],"genre_scores_gemma":[0.9112664,0.001406289,0.07858577,0.0001065242,0.00002994909,0.000112521,0.0002868894,0.00006218149,0.008143391],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008887037,"threshold_uncertainty_score":0.004007638,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02322420504039401,"score_gpt":0.2815921888691981,"score_spread":0.2583679838288041,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}