{"id":"W4211082835","doi":"10.1039/d2an00145d","title":"Homogeneous assays for aptamer-based ethanolamine sensing: no indication of target binding","year":2022,"lang":"en","type":"article","venue":"The Analyst","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada; Mitacs","keywords":"Ethanolamine; Aptamer; Chemistry; Dissociation constant; Binding constant; Isothermal titration calorimetry; Fluorescence; Binding site; Biochemistry; Biology; Molecular biology; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006448396,0.0009005132,0.0003844676,0.000445656,0.0002399594,0.00054399,0.0006874304,0.001160761,0.001698159],"category_scores_gemma":[0.0008903124,0.0004069131,0.000204841,0.0002646125,0.0003693135,0.0004503995,0.0003636357,0.0008409022,0.001223638],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003745154,"about_ca_system_score_gemma":0.0002771396,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006554027,"about_ca_topic_score_gemma":0.002256249,"domain_scores_codex":[0.9987349,0.0003302458,0.00009267651,0.0003685331,0.0003781518,0.00009552076],"domain_scores_gemma":[0.9992254,0.0003907335,0.0001117015,0.00006363825,0.0001502417,0.00005828379],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001314037,0.00002000338,0.0001258705,0.00006024033,0.000007272378,0.00002850052,0.00001977922,0.00002888982,0.9985386,0.0000522757,0.00003448738,0.001071059],"study_design_scores_gemma":[0.000002810563,0.00006769845,0.000732051,0.000006773329,0.00001171677,0.00008457652,0.00001862692,0.0005121279,0.9978181,0.00004338949,0.0006988436,0.000003125399],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7328628,0.01250562,0.2407305,0.000473473,0.0003381419,0.0003444692,0.0006616063,0.00101701,0.01106638],"genre_scores_gemma":[0.9168192,0.002534366,0.07022873,0.0003933693,0.00005478656,0.0002607325,0.0006781276,0.00007371669,0.008957014],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001698159,"threshold_uncertainty_score":0.005680919,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01178991193348531,"score_gpt":0.2637721420930978,"score_spread":0.2519822301596125,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}