{"id":"W4211082835","doi":"10.1039/d2an00145d","title":"Homogeneous assays for aptamer-based ethanolamine sensing: no indication of target binding","year":2022,"lang":"en","type":"article","venue":"The Analyst","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada; Mitacs","keywords":"Ethanolamine; Aptamer; Chemistry; Dissociation constant; Binding constant; Isothermal titration calorimetry; Fluorescence; Binding site; Biochemistry; Biology; Molecular biology; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000490074,0.0001088587,0.0001656532,0.0000971176,0.0002470491,0.000008145949,0.0001982448,0.0000526412,0.000004745303],"category_scores_gemma":[0.00007872345,0.00008389733,0.0001865594,0.0002690267,0.00008386883,0.000001342701,0.00008808977,0.00007959895,8.008215e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002259797,"about_ca_system_score_gemma":0.00004394617,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001466704,"about_ca_topic_score_gemma":0.000007491467,"domain_scores_codex":[0.9991341,0.0001046206,0.0002129768,0.0002325254,0.0001615471,0.0001542408],"domain_scores_gemma":[0.9991629,0.0000322033,0.0002494559,0.0004037624,0.0001276735,0.00002405166],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008684629,0.00004648832,0.0002305229,0.000009142875,0.0001178926,9.103066e-7,0.0000126662,0.0004957854,0.9966412,0.00002389322,0.0007066924,0.001627986],"study_design_scores_gemma":[0.0002044581,0.0003385585,0.00003333326,0.000003854419,0.0001254126,0.000007338086,0.00008896575,0.007090676,0.9716212,0.0001007223,0.02025279,0.0001326983],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.931227,0.0002509939,0.06699187,0.0005561209,0.00007153393,0.0003126859,0.0001918765,0.00003902088,0.0003588709],"genre_scores_gemma":[0.9868897,0.00001605008,0.01170065,0.0002157458,0.00009689672,0.0000133301,0.0006543345,0.00001679763,0.0003965427],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05566262,"threshold_uncertainty_score":0.3421234,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01178991193348531,"score_gpt":0.2637721420930978,"score_spread":0.2519822301596125,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}