{"id":"W4212816792","doi":"10.1007/978-1-0716-2075-5_3","title":"Llama DNA Immunization and Isolation of Functional Single-Domain Antibody Binders","year":2022,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":9,"is_retracted":false,"has_abstract":false,"ca_institutions":"Carleton University; University of Ottawa; Natural Sciences and Engineering Research Council of Canada; National Research Council Canada","funders":"","keywords":"Immunization; Immunogenicity; Gene gun; Naked DNA; Biology; Monoclonal antibody; Phage display; Antibody; Virology; Immune system; Immunology; Genetic enhancement; Computational biology; DNA vaccination; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003405993,0.0004894135,0.000583065,0.0005384191,0.0003243217,0.0005389475,0.0005742627,0.0005289523,0.004550261],"category_scores_gemma":[0.000550779,0.0003571924,0.0004217126,0.0004451664,0.0002535917,0.0002773093,0.0004896977,0.001449408,0.002050501],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005698294,"about_ca_system_score_gemma":0.000265184,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007025345,"about_ca_topic_score_gemma":0.001176013,"domain_scores_codex":[0.9996617,0.00004266576,0.00002611645,0.0000867377,0.00008088035,0.0001018816],"domain_scores_gemma":[0.9997362,0.00007883262,0.00004165322,0.00005284734,0.00004804652,0.00004231695],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005658746,0.00003144794,0.0001417465,0.00002984647,0.000006594482,0.00002932518,0.0000271054,0.0001201861,0.9961215,0.0002380009,0.000138881,0.003058604],"study_design_scores_gemma":[0.00001601735,0.00007988501,0.0008556796,0.000006828246,0.00001274149,0.0000973432,0.00001786611,0.001015882,0.9898815,0.00007090173,0.007940657,0.000004641992],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8965238,0.001924057,0.09045096,0.0005257183,0.0001494657,0.0003012179,0.001935358,0.0009866252,0.007202766],"genre_scores_gemma":[0.9288741,0.001325957,0.04429267,0.0003566287,0.00004722051,0.0003437275,0.005063546,0.0002908951,0.01940533],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.004550261,"threshold_uncertainty_score":0.01522207,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0398715632896056,"score_gpt":0.411754926164839,"score_spread":0.3718833628752334,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}