{"id":"W4212863247","doi":"10.1002/bit.28068","title":"Only a small fraction of cells produce assembled capsids during transfection‐based manufacturing of adeno‐associated virus vectors","year":2022,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; McGill University","funders":"National Research Council Canada","keywords":"Transfection; Capsid; Adeno-associated virus; Flow cytometry; Plasmid; Vector (molecular biology); Titer; Virus; Recombinant DNA; Biology; Cell biology; Molecular biology; Chemistry; Virology; Cell culture; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002404368,0.000154474,0.0002144328,0.000286308,0.0001236465,0.000004508579,0.0001750292,0.0002516236,0.00001879159],"category_scores_gemma":[0.00002329091,0.0001718406,0.00007609399,0.0002373386,0.00006527412,0.000003829177,0.0000939262,0.0002985252,3.459835e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005014004,"about_ca_system_score_gemma":0.00006242224,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005751123,"about_ca_topic_score_gemma":0.00009299316,"domain_scores_codex":[0.9990171,0.00006057122,0.0002283254,0.0003163425,0.0001271063,0.000250549],"domain_scores_gemma":[0.999565,0.00001337701,0.00009209664,0.0002625771,0.0000317338,0.00003518212],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005207849,0.0001092242,0.0006664083,0.00009268095,0.0001268814,0.000003483719,0.00001973368,0.004204398,0.9934945,0.000003620341,0.000003569377,0.0007547446],"study_design_scores_gemma":[0.0006920877,0.00153037,0.002723237,0.00001534408,0.00002373118,0.00001242323,0.00007249958,0.0003945298,0.9942559,0.000003825767,0.0001182604,0.0001577823],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9980029,0.0006295919,0.0008461252,0.00009624922,0.00009746477,0.0002275955,0.00003526269,0.00005914151,0.000005619795],"genre_scores_gemma":[0.9995214,0.0001646343,0.000177039,0.000008762984,0.00002550357,0.00002927252,0.00002507554,0.00002682391,0.00002151379],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003809868,"threshold_uncertainty_score":0.7007457,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00850697074966471,"score_gpt":0.2210072267209617,"score_spread":0.212500255971297,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}