{"id":"W4212863247","doi":"10.1002/bit.28068","title":"Only a small fraction of cells produce assembled capsids during transfection‐based manufacturing of adeno‐associated virus vectors","year":2022,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; McGill University","funders":"National Research Council Canada","keywords":"Transfection; Capsid; Adeno-associated virus; Flow cytometry; Plasmid; Vector (molecular biology); Titer; Virus; Recombinant DNA; Biology; Cell biology; Molecular biology; Chemistry; Virology; Cell culture; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009822568,0.0004978029,0.0008494014,0.0003566764,0.0003193835,0.001295823,0.0002491757,0.0003664021,0.001136872],"category_scores_gemma":[0.0009450228,0.0002823316,0.0003584211,0.000347546,0.0004509176,0.000557504,0.0003761951,0.0006089055,0.001598039],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004051663,"about_ca_system_score_gemma":0.0003838692,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007008463,"about_ca_topic_score_gemma":0.0007761192,"domain_scores_codex":[0.9992422,0.0001255263,0.00008369947,0.0001815985,0.0002843458,0.00008268072],"domain_scores_gemma":[0.9993061,0.0003230903,0.000095259,0.0001062527,0.0001328863,0.00003637783],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004346017,0.00002058004,0.0008997718,0.00004039618,0.000007237375,0.000047111,0.00008230877,0.0001571537,0.9949841,0.0001823408,0.00005314562,0.003482347],"study_design_scores_gemma":[0.000005337244,0.000134414,0.00332998,0.000007608866,0.00001304488,0.0001604241,0.00004632272,0.001190843,0.991595,0.0001155205,0.003395291,0.00000630713],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8121932,0.002337701,0.1772881,0.0002122944,0.00009131679,0.0003550431,0.000892914,0.0006953302,0.005934191],"genre_scores_gemma":[0.9434192,0.00180375,0.04605373,0.0002594131,0.00003351993,0.000274328,0.001935117,0.0002386148,0.00598229],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001295823,"threshold_uncertainty_score":0.005194724,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00850697074966471,"score_gpt":0.2210072267209617,"score_spread":0.212500255971297,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}