{"id":"W4214712588","doi":"10.1101/2022.02.25.482014","title":"Generation of PRKN and PINK1-KO and double KO cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing","year":2022,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Neurological Institute and Hospital","funders":"Canadian Institutes of Health Research; Canada First Research Excellence Fund; McGill University; Québec Consortium for Drug Discovery; Michael J. Fox Foundation for Parkinson's Research","keywords":"PINK1; Induced pluripotent stem cell; CRISPR; Biology; Ubiquitin ligase; Cell biology; Genetics; Embryonic stem cell; Mitophagy; Ubiquitin; Gene; Autophagy; Apoptosis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005242224,0.0004239815,0.0003639395,0.000543948,0.0002965268,0.0005440364,0.0005285464,0.0005880026,0.003611228],"category_scores_gemma":[0.0003073124,0.0002789353,0.0004034162,0.0003068466,0.0004357932,0.0002764261,0.0005626128,0.001010525,0.001724291],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002693328,"about_ca_system_score_gemma":0.0002682211,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006771497,"about_ca_topic_score_gemma":0.001026636,"domain_scores_codex":[0.9995736,0.00002937931,0.00007976229,0.0001110371,0.0001597105,0.00004654721],"domain_scores_gemma":[0.9997045,0.00005078697,0.00005409298,0.00007588208,0.00004066012,0.00007401592],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001196963,0.00004070341,0.0003181116,0.00005592686,0.00001681182,0.0002248616,0.00005729934,0.0002288902,0.9948151,0.0009332943,0.0004771973,0.002712216],"study_design_scores_gemma":[0.00005680746,0.00008799938,0.001940017,0.00001157405,0.0000331949,0.001023796,0.00003496883,0.001942251,0.9819009,0.0001823043,0.01277042,0.00001582128],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8481829,0.001195712,0.1184882,0.0005018197,0.0005662108,0.001148382,0.01688788,0.001560158,0.01146871],"genre_scores_gemma":[0.9114991,0.0008379726,0.047988,0.0002493455,0.00003355939,0.0008378424,0.01325602,0.0006988827,0.02459933],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003611228,"threshold_uncertainty_score":0.01208079,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05110329490863515,"score_gpt":0.2748818571227971,"score_spread":0.223778562214162,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}