{"id":"W4214919916","doi":"10.1016/j.ymeth.2022.03.002","title":"Structure-function analysis of DEAD-box helicase DDX43","year":2022,"lang":"en","type":"article","venue":"Methods","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Helicase; RNA Helicase A; Processivity; DEAD box; Biology; Cell biology; Chromatin immunoprecipitation; Electrophoretic mobility shift assay; Immunoprecipitation; AAA proteins; Molecular biology; Chemistry; DNA; Biochemistry; Gene; ATPase; RNA; Transcription factor; DNA replication; Gene expression; Promoter; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002254082,0.0003372256,0.0004407531,0.0002515588,0.000247624,0.0002468617,0.0002394584,0.0003930625,0.001290208],"category_scores_gemma":[0.0002054689,0.0001463983,0.000481242,0.0003147604,0.0001337747,0.0002211857,0.0002120988,0.0004378544,0.0004863513],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003030165,"about_ca_system_score_gemma":0.0001786911,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001037877,"about_ca_topic_score_gemma":0.0009170955,"domain_scores_codex":[0.9998975,0.00001157324,0.000009885794,0.00003127362,0.00003688516,0.00001288486],"domain_scores_gemma":[0.999905,0.00001691817,0.00002073192,0.000005159603,0.00002260689,0.00002953583],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002418801,0.00007263771,0.002185011,0.0001705866,0.00002899307,0.0003725254,0.00008914836,0.001205947,0.9918104,0.0002573291,0.0002719259,0.00329366],"study_design_scores_gemma":[0.00008747556,0.0006552939,0.05422847,0.00003897938,0.000114094,0.001706598,0.0002407971,0.04579628,0.8803009,0.0003368387,0.01643545,0.00005871969],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9921471,0.002276643,0.003197149,0.0001270236,0.00003398116,0.00002328896,0.001320983,0.00007787688,0.0007959788],"genre_scores_gemma":[0.9846253,0.001404957,0.006051723,0.00007274766,0.000008986578,0.00002405704,0.005531335,0.00005198655,0.002228905],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001290208,"threshold_uncertainty_score":0.004316151,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02189030755057999,"score_gpt":0.3811296424520196,"score_spread":0.3592393349014397,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}