{"id":"W4220662353","doi":"10.1101/2022.03.17.484691","title":"Establishment of an efficient transformation and CRISPR/Cas9-mediated gene editing system in Chinese local planting cassava ( <i>Manihot esculenta</i> Crantz) cultivar SC8","year":2022,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Cassava research and cyanide","field":"Agricultural and Biological Sciences","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Earmarked Fund for China Agriculture Research System; Institute of Genetics and Developmental Biology, Chinese Academy of Sciences; Chinese Academy of Sciences; Institute of Genetics; National Natural Science Foundation of China","keywords":"Agrobacterium; Acetosyringone; Manihot esculenta; Cultivar; Transformation (genetics); Biology; Gene; Genetically modified crops; Horticulture; CRISPR; Transformation efficiency; Strain (injury); Botany; Transgene; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002329532,0.0003783202,0.0003664274,0.0003506816,0.0002909906,0.0002903808,0.0003984608,0.0002378468,0.0009418771],"category_scores_gemma":[0.0001361696,0.0002135057,0.0003155009,0.0002884631,0.00014267,0.0001328404,0.0004295754,0.0004527077,0.0008096606],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000273669,"about_ca_system_score_gemma":0.0003094285,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001943834,"about_ca_topic_score_gemma":0.00246313,"domain_scores_codex":[0.999845,0.00001326038,0.00002394004,0.00004516373,0.00004650606,0.00002603763],"domain_scores_gemma":[0.99993,0.000008873696,0.00001662536,0.00001711858,0.00001181477,0.0000156068],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001851614,0.000007837867,0.0001576824,0.00002534272,0.000003328137,0.00007928786,0.00002291726,0.00004426893,0.9981919,0.00007711015,0.00005118824,0.001320545],"study_design_scores_gemma":[0.00001938247,0.0001036896,0.004846997,0.00001120714,0.00003707372,0.00067928,0.00005858415,0.002207277,0.9820521,0.00006890811,0.009897252,0.00001802837],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.959415,0.0004792058,0.03381889,0.0001198936,0.00006524583,0.000250545,0.001899014,0.0007276683,0.003224551],"genre_scores_gemma":[0.9409171,0.000654412,0.03933049,0.00005124817,0.00001292602,0.0002464912,0.006586465,0.0002774406,0.01192346],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001943834,"threshold_uncertainty_score":0.003865004,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01131500978662979,"score_gpt":0.2169514449791944,"score_spread":0.2056364351925646,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}