{"id":"W4220664868","doi":"10.1021/acs.biomac.1c01191","title":"Extracellular Secretion and Simple Purification of Bacterial Collagen from <i>Escherichia coli</i>","year":2022,"lang":"en","type":"article","venue":"Biomacromolecules","topic":"Collagen: Extraction and Characterization","field":"Materials Science","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Fonds de recherche du Québec – Nature et technologies; Canada Foundation for Innovation; Natural Sciences and Engineering Research Council of Canada; Faculty of Engineering, McGill University","keywords":"Secretion; Lysis; Extracellular; Recombinant DNA; Chemistry; Biochemistry; Extracellular matrix; Escherichia coli; Intracellular; Biophysics; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003630503,0.0006440075,0.0002992372,0.0002562125,0.0002807201,0.0004383936,0.0002612169,0.0003464529,0.0005482211],"category_scores_gemma":[0.0003381333,0.0001688117,0.0002590693,0.0003007003,0.0002838514,0.0001928633,0.0003222782,0.0006034051,0.0009618724],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003005516,"about_ca_system_score_gemma":0.0004202244,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001035957,"about_ca_topic_score_gemma":0.001610733,"domain_scores_codex":[0.9995244,0.00008761463,0.00004689584,0.00009081714,0.0001910559,0.00005922899],"domain_scores_gemma":[0.9998071,0.00002617169,0.00006285151,0.00002701736,0.00005014483,0.00002672629],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000009562103,0.000008532088,0.000084176,0.00004401664,0.000001900889,0.00004194409,0.0000120983,0.00003386082,0.9985365,0.00004381728,0.00004547317,0.00113812],"study_design_scores_gemma":[0.000003791796,0.00004070248,0.001244756,0.00001087202,0.000005745257,0.0003420757,0.00001117616,0.0004082138,0.9914111,0.00002624083,0.006488128,0.000007096665],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7417156,0.007371579,0.2384229,0.0007246452,0.0002179273,0.00083299,0.003566944,0.001137225,0.00601009],"genre_scores_gemma":[0.7496849,0.007175783,0.2264487,0.0004353191,0.00009057476,0.0005393915,0.007819375,0.0003666477,0.00743933],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001035957,"threshold_uncertainty_score":0.002180696,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.011348594616973,"score_gpt":0.2204437735906317,"score_spread":0.2090951789736587,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}