{"id":"W4220722511","doi":"10.1039/d2cc00792d","title":"Dual-amplified CRISPR-Cas12a bioassay for HIV-related nucleic acids","year":2022,"lang":"en","type":"article","venue":"Chemical Communications","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":29,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Fundamental Research Fund of Shandong University; Sichuan University; Department of Science and Technology of Sichuan Province; National Natural Science Foundation of China","keywords":"Nucleic acid; CRISPR; Bioassay; DNA; Nucleic Acid Amplification Tests; Human immunodeficiency virus (HIV); Computational biology; Chemistry; Combinatorial chemistry; Biology; Molecular biology; Nanotechnology; Chromatography; Biochemistry; Genetics; Gene; Materials science; Virology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00016004,0.0001224064,0.0001172857,0.00003445934,0.0003063423,0.00001645895,0.0006597431,0.00008816126,0.00006098306],"category_scores_gemma":[0.0001088121,0.0001421427,0.0001244625,0.0001430629,0.00009747809,0.000002046931,0.000836883,0.0002114524,0.00001113135],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002998322,"about_ca_system_score_gemma":0.00003799113,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003980622,"about_ca_topic_score_gemma":0.000002062758,"domain_scores_codex":[0.9991882,0.00004361966,0.0002299185,0.0002362684,0.00008776898,0.0002142684],"domain_scores_gemma":[0.9984783,0.00005844378,0.00004865144,0.001280765,0.00005645596,0.00007736651],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00002001807,0.0001066507,0.0001007242,0.000009642643,0.00004697363,2.133643e-7,0.00008817801,0.0001741884,0.9853498,0.001213253,0.01203627,0.0008540578],"study_design_scores_gemma":[0.0006793374,0.00007083158,0.0001121388,0.000003823339,0.00003708229,0.00002131786,0.000222624,0.00159823,0.4228857,0.0004904078,0.5736243,0.0002542136],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9070079,0.01099443,0.04929095,0.01324863,0.0005830466,0.001708732,0.000961356,0.0003218458,0.01588311],"genre_scores_gemma":[0.9893992,0.0001087306,0.007429332,0.0002238407,0.00005274936,0.0003442522,0.001591449,0.00003502687,0.0008154297],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.5624642,"threshold_uncertainty_score":0.579641,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01940045019359637,"score_gpt":0.317894992915263,"score_spread":0.2984945427216666,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}