{"id":"W4220760958","doi":"10.1016/j.jbc.2022.101615","title":"Can two wrongs make a right? F508del-CFTR ion channel rescue by second-site mutations in its transmembrane domains","year":2022,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Cystic Fibrosis Research Advances","field":"Medicine","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Medical Research Council; Natural Sciences and Engineering Research Council of Canada; University College Cork; Canadian Institutes of Health Research; Sparks; Canada Research Chairs; University College London; University of Cambridge; Cystic Fibrosis Trust; Compute Canada","keywords":"Cystic fibrosis transmembrane conductance regulator; Transmembrane domain; Chemistry; Hydrogen bond; Biophysics; Transmembrane protein; ATP-binding cassette transporter; Mutagenesis; Cyclic nucleotide-binding domain; Protein structure; Mutation; Crystallography; Biochemistry; Peptide sequence; Membrane; Biology; Transporter; Molecule","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007439039,0.0003178444,0.0007036495,0.0002006317,0.0008541719,0.0007725974,0.0005442167,0.001969724,0.002284123],"category_scores_gemma":[0.002035609,0.0002522685,0.0006244729,0.0001402597,0.001206593,0.001838496,0.001016326,0.002179942,0.0007419816],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003839588,"about_ca_system_score_gemma":0.0006415365,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001103649,"about_ca_topic_score_gemma":0.001489794,"domain_scores_codex":[0.99968,0.0000615137,0.00002369218,0.0000611022,0.00009380784,0.00007990455],"domain_scores_gemma":[0.9994584,0.0001751584,0.0000862705,0.000088122,0.00005003731,0.0001421016],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.004113265,0.0007391665,0.01136556,0.0005915213,0.0005718191,0.01421295,0.002297953,0.01598588,0.8177012,0.05361268,0.01954474,0.05926331],"study_design_scores_gemma":[0.001340682,0.002899516,0.02372737,0.00120105,0.0006548564,0.02462334,0.00850929,0.09603879,0.4660818,0.1404052,0.2336186,0.000899577],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9534405,0.004962486,0.01195801,0.01728973,0.002278534,0.00004195671,0.0002833034,0.0005030124,0.00924252],"genre_scores_gemma":[0.9859038,0.001458593,0.005089401,0.002605821,0.00008028161,0.00002184084,0.0001770726,0.0001425144,0.004520605],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002284123,"threshold_uncertainty_score":0.007641137,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01883097414641147,"score_gpt":0.2964939196453188,"score_spread":0.2776629454989073,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}