{"id":"W4220842621","doi":"10.1128/jvi.01929-21","title":"Detection of the HIV-1 Accessory Proteins Nef and Vpu by Flow Cytometry Represents a New Tool to Study Their Functional Interplay within a Single Infected CD4 <sup>+</sup> T Cell","year":2022,"lang":"en","type":"article","venue":"Journal of Virology","topic":"HIV Research and Treatment","field":"Immunology and Microbiology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; McGill University; Centre Hospitalier de l’Université de Montréal","funders":"National Institute of Allergy and Infectious Diseases; Canadian Institutes of Health Research; Government of Canada; National Institutes of Health; Canada Foundation for Innovation; Canada Research Chairs","keywords":"Antibody-dependent cell-mediated cytotoxicity; Biology; Cell biology; Flow cytometry; Virology; Epitope; Viral replication; Immune system; Intracellular; Antibody; Virus; Monoclonal antibody; Molecular biology; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004678413,0.0005460799,0.0005484076,0.0007001808,0.0004148123,0.0008452313,0.0004742909,0.0008726265,0.001413192],"category_scores_gemma":[0.0004889358,0.0002137236,0.0002194201,0.0002403088,0.0005957644,0.001037147,0.0004714365,0.001273213,0.0003404177],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004000226,"about_ca_system_score_gemma":0.0002680189,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003360946,"about_ca_topic_score_gemma":0.0005163884,"domain_scores_codex":[0.9996755,0.00006932768,0.00002147401,0.00009919431,0.00008171797,0.00005278047],"domain_scores_gemma":[0.9995543,0.0002560108,0.00005066576,0.00004679145,0.00003926432,0.00005286797],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003618056,0.00001264304,0.0002979945,0.00002108689,0.000004008042,0.00001608086,0.00002040108,0.00003359709,0.996573,0.000334672,0.00002561064,0.002624652],"study_design_scores_gemma":[0.00001071358,0.0001365521,0.002141443,0.000006423352,0.00001339721,0.0003752848,0.00003864777,0.00314893,0.9914269,0.0005572685,0.002132124,0.00001236494],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8232116,0.002930396,0.1690326,0.0004670905,0.00009836777,0.0001545822,0.0004657925,0.0005196581,0.003120103],"genre_scores_gemma":[0.866816,0.001637684,0.128799,0.0001936964,0.00008987057,0.0002879821,0.0003520334,0.0000581124,0.001765501],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001413192,"threshold_uncertainty_score":0.004727602,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01382347340007956,"score_gpt":0.2484152462833609,"score_spread":0.2345917728832813,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}